Abstract
Breakthrough gastrointestinal COVID-19 was observed after experimental SARS-CoV-2 upper mucosal infection in a rhesus macaque undergoing low-dose monoclonal antibody prophylaxis. High levels of viral RNA were detected in intestinal sites contrasting with minimal viral replication in upper respiratory mucosa. Sequencing of virus recovered from tissue in 3 gastrointestinal sites and rectal swab revealed loss of furin cleavage site deletions present in the inoculating virus stock and 2 amino acid changes in spike that were detected in 2 colon sites but not elsewhere, suggesting compartmentalized replication and intestinal viral evolution. This suggests suboptimal antiviral therapies promote viral sequestration in these anatomies.
Keywords: SARS-CoV-2; breakthrough infection; gastrointestinal tract; nonhuman primates; suboptimal prophylaxis.
【저자키워드】 SARS-CoV-2, Breakthrough infection, gastrointestinal tract, nonhuman primates, suboptimal prophylaxis., 【초록키워드】 COVID-19, antiviral therapy, monoclonal antibody, Infection, virus, Prophylaxis, Replication, viral replication, viral evolution, furin cleavage site, low-dose, Deletion, Viral RNA, mucosal, mucosa, Colon, tissue, rhesus, rectal swab, intestinal, upper respiratory, amino acid change, promote, virus stock, 【제목키워드】 COVID-19, Gastrointestinal, Consequent,