Abstract
During the COVID-19 (coronavirus disease 2019) pandemic, several SARS-CoV-2 variants of concern emerged, including the Omicron variant, which has enhanced infectivity and immune invasion. Many antibodies and aptamers that bind the spike (S) of previous strains of SARS-CoV-2 either do not bind or bind with low affinity to Omicron S. In this study, we report a high-affinity S ARS- C oV-2 O micron R BD-binding aptam e r (SCORe) that binds Omicron BA.1 and BA.2 RBD with nanomolar K D1 . We employ aptamers SCORe.50 and SNAP4.74 in a multiplexed lateral flow assay (LFA) to distinguish between Omicron and wild-type S at concentrations as low as 100 pM. Finally, we show that SCORe.50 and its dimerized form SCOReD can neutralize Omicron S-pseudotyped virus infection of ACE2-overexpressing cells by >70%. SCORe therefore has potential applications in COVID-19 rapid diagnostics as well as in viral neutralization.
【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, pandemic, antibody, neutralization, variant, SARS-CoV-2 variant, omicron, diagnostics, immune, lateral flow, RBD, virus infection, Concentration, strain, Invasion, wild-type, Cell, neutralize, bind, in viral, dimerized, 【제목키워드】 SARS-CoV-2, detection, omicron, aptamer, DNA, Rapid,