Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally since December 2019. Several studies reported that SARS-CoV-2 infections may produce false-positive reactions in dengue virus (DENV) serology tests and vice versa. However, it remains unclear whether SARS-CoV-2 and DENV cross-reactive antibodies provide cross-protection against each disease or promote disease severity. In this study, we confirmed that antibodies against the SARS-CoV-2 spike protein and its receptor-binding domain (S1-RBD) were significantly increased in dengue patients compared to normal controls. In addition, anti-S1-RBD IgG purified from S1-RBD hyperimmune rabbit sera could cross-react with both DENV envelope protein (E) and nonstructural protein 1 (NS1). The potential epitopes of DENV E and NS1 recognized by these antibodies were identified by a phage-displayed random peptide library. In addition, DENV infection and DENV NS1-induced endothelial hyperpermeability in vitro were inhibited in the presence of anti-S1-RBD IgG. Passive transfer anti-S1-RBD IgG into mice also reduced prolonged bleeding time and decreased NS1 seral level in DENV-infected mice. Lastly, COVID-19 patients’ sera showed neutralizing ability against dengue infection in vitro . Thus, our results suggest that the antigenic cross-reactivity between the SARS-CoV-2 S1-RBD and DENV can induce the production of anti-SARS-CoV-2 S1-RBD antibodies that cross-react with DENV which may hinder dengue pathogenesis.
Keywords: COVID-19; SARS-CoV-2; antibody-dependent enhancement; dengue virus; diagnosis.
【저자키워드】 COVID-19, SARS-CoV-2, Antibody-dependent enhancement, dengue virus, diagnosis., 【초록키워드】 IgG, coronavirus, Pathogenesis, cross-protection, antibody, SARS-COV-2 infection, disease severity, Infection, peptide, Diagnosis, in vitro, anti-SARS-CoV-2, Spike protein, cross-reactivity, Spread, mice, sera, bleeding, envelope protein, serology test, Neutralizing, epitope, disease, False-positive, nonstructural protein 1, COVID-19 patient, cross-reactive antibody, passive transfer, endothelial, acute respiratory syndrome, reaction, antigenic, domain, random, controls, hyperimmune, significantly increased, reported, addition, inhibited, reduced, promote, induce, purified, cross-react, dengue patient, the SARS-CoV-2, 【제목키워드】 Pathogenesis, cross-react, the SARS-CoV-2,