Abstract
Multisystem inflammatory syndrome in children (MIS-C) can complicate infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but differences in the immune responses during MIS-C compared to coronavirus disease 2019 (COVID-19) are poorly understood. We longitudinally compared the amounts and avidity of plasma anti-nucleocapsid (N) and spike (S) antibodies, phenotypes of B cells, and numbers of virus-specific antibody-secreting cells in circulation of children hospitalized with COVID-19 (n = 10) and with MIS-C (n = 12). N-specific immunoglobulin G (IgG) was higher early after presentation for MIS-C than COVID-19 patients and avidity of N- and S-specific IgG at presentation did not mature further during follow-up as it did for COVID-19. Both groups had waning proportions of B cells in circulation and decreasing but sustained production of virus-specific antibody-secreting cells for months. Overall, B-cell responses were similar, but those with MIS-C demonstrated a more mature antibody response at presentation compared to COVID-19, suggesting a postinfectious entity.
Keywords: COVID-19; antibody avidity; antibody-secreting cells; antiviral antibody; flow cytometry.
【저자키워드】 COVID-19, flow cytometry, Antibody avidity, antibody-secreting cells, antiviral antibody, 【초록키워드】 coronavirus disease, antibodies, SARS-CoV-2, IgG, coronavirus, immune response, Hospitalized, Antiviral, antibody, children, Antibody Response, Infection, B cells, flow cytometry, MIS-C, B cell, Immunoglobulin, phenotype, plasma, Follow-up, circulation, group, antibody-secreting cell, Inflammatory, COVID-19 patient, Postinfectious, acute respiratory syndrome, syndrome, B-cell response, proportion, demonstrated, sustained, complicate, those with MIS-C, with COVID-19, 【제목키워드】 response, respiratory,