Abstract
Safe, passive immunization methods are required against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants. Immunization of chickens with antigen is known to induce specific IgY antibodies concentrated in the egg yolk and has a good safety profile, high yield of IgY per egg, can be topically applied, not requiring parenteral delivery. Our data provide the first evidence of the prophylactic efficacy of Immunoglobulin Y antibodies against SARS-CoV-2 in mice. Lohmann hens were injected with recombinant SARS-CoV-2 RBD protein; IgY-Abs were extracted from the eggs and characterized using SDS-PAGE. Antiviral activity was evaluated using plaque reduction neutralization tests. In additional experiments, IgY-RBD efficacy was examined in mice sensitized to SARS-CoV-2 infection by transduction with Ad5-hACE2 (mild disease) or by using mouse-adapted virus (severe disease). In both cases, prophylactic intranasal administration of IgY-Abs reduced SARS-CoV-2 replication, and reduced morbidity, inflammatory cell infiltration, hemorrhage, and edema in the lungs and increased survival compared to control groups that received non-specific IgY-Abs. These results indicate that further evaluation of IgY-RBD antibodies in humans is warranted.
【초록키워드】 SARS-CoV-2, Efficacy, antibody, SARS-COV-2 infection, Human, lung, Prophylactic, virus, immunization, variants, Antigen, mice, morbidity, edema, Mild, disease, SARS-CoV-2 replication, non-specific, safety profile, intranasal, Coronavirus-2, Evidence, administration, severe disease, hemorrhage, acute respiratory syndrome, control group, increased survival, Recombinant SARS-CoV-2 RBD, inflammatory cell infiltration, plaque reduction neutralization, SDS-PAGE, Ad5-hACE2, examined, evaluated, required, injected, applied, reduced, characterized, induce, experiments, 【제목키워드】 antibody, Immunoglobulin, mice, Protective,