Abstract
Kawasaki disease (KD), a multisystem inflammatory syndrome that occurs in children, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or COVID-19) may share some overlapping mechanisms. The purpose of this study was to analyze the differences in single-cell RNA sequencing between KD and COVID-19. We performed single-cell RNA sequencing in KD patients (within 24 hours before IVIG treatment) and age-matched fever controls. The single-cell RNA sequencing data of COVID-19, influenza, and health controls were downloaded from the Sequence Read Archive (GSE149689/PRJNA629752). In total, 22 single-cell RNA sequencing data with 102,355 nuclei were enrolled in this study. After performing hierarchical and functional clustering analyses, two enriched gene clusters demonstrated similar patterns in severe COVID-19 and KD, heightened neutrophil activation, and decreased MHC class II expression. Furthermore, comparable dysregulation of neutrophilic granulopoiesis representing two pronounced hyperinflammatory states was demonstrated, which play a critical role in the overactivated and defective aging program of granulocytes, in patients with KD as well as those with severe COVID-19. In conclusion, both neutrophil activation and MHC class II reduction play a crucial role and thus may provide potential treatment targets for KD and severe COVID-19.
Keywords: COVID-19; aged neutrophils; kawasaki disease; overactivation; single-cell RNA sequencing.
【저자키워드】 COVID-19, Kawasaki disease, single-cell RNA sequencing., overactivation, aged neutrophils, 【초록키워드】 Treatment, SARS-CoV-2, coronavirus, severe COVID-19, Influenza, children, Single-cell RNA sequencing, Health, Fever, Patient, Clustering, Control, target, mechanisms, expression, Critical, hyperinflammatory state, Inflammatory, Potential treatment, neutrophil activation, dysregulation, reduction, overlapping, Hierarchical, acute respiratory syndrome, syndrome, neutrophilic, gene cluster, MHC class, Sequence Read Archive, controls, nuclei, enrolled, performed, functional, occur, demonstrated, comparable, representing, analyses, 24 hour, downloaded, 【제목키워드】 severe COVID-19, neutrophil, bidirectional, immune dysregulation,