Abstract
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments. Moreover, the possibility of the appearance of a new betacoronavirus with high transmissibility and high fatality is reason for concern. In this study, we used a natively paired yeast display technology, combined with next-generation sequencing (NGS) and massive bioinformatic analysis to perform a comprehensive study of subdomain specificity of natural human antibodies from two convalescent donors. Using this screening technology, we mapped the cross-reactive responses of antibodies generated by the two donors against SARS-CoV-2 variants and other betacoronaviruses. We tested the neutralization potency of a set of the cross-reactive antibodies generated in this study and observed that most of the antibodies produced by these patients were non-neutralizing. We performed a comparison of the specific and non-specific antibodies by somatic hypermutation in a repertoire-scale for the two individuals and observed that the degree of somatic hypermutation was unique for each patient. The data from this study provide functional insights into cross-reactive antibodies that can assist in the development of strategies against emerging SARS-CoV-2 variants and divergent betacoronaviruses.
Keywords: SARS-CoV-2; SARS-CoV-2 variants; betacoronaviruses; cross-reactive antibodies; yeast display.
【저자키워드】 SARS-CoV-2, SARS-CoV-2 variants, cross-reactive antibodies, betacoronaviruses, yeast display., 【초록키워드】 coronavirus, Vaccines, antibody, NGS, SARS-CoV-2 variant, diagnostics, Betacoronavirus, specificity, Transmissibility, response, Next-generation sequencing, Patient, Effectiveness, donors, convalescent, non-specific, yeast, Donor, fatality, cross-reactive, cross-reactive antibody, acute respiratory syndrome, individual, neutralization potency, bioinformatic analysis, produced, tested, performed, functional, unique, mapped, assist, natural human antibody, the antibody, 【제목키워드】 coronavirus, Antigen, anti-SARS-CoV-2 antibody, monoclonal, mapping,