Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.
【저자키워드】 breath, 【초록키워드】 COVID-19, immunology, Vaccine development, immune response, Infectious diseases, Diseases, Vaccines, Cellular immune response, Infectious disease, Prophylactic, Single-cell RNA sequencing, immune responses, RNA sequencing, vaccine platform, Immunological response, power, regimens, cellular immune responses, offer, widespread, example, highlighting, used to evaluate, 【제목키워드】 RNA, application,