Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein mediates membrane fusion between the virus and the target cells, triggering viral entry into the latter. Here, we describe a SARS-CoV-2 spike-protein-mediated membrane fusion assay using a dual functional split reporter protein to quantitatively monitor the fusion kinetics of the viral and target cell membranes in living cells. This approach can be applied in various cell types, potentially predicting the pathogenicity of newly emerging variants. For complete details on the use and execution of this protocol, please refer to Kimura et al. (2022b), Kimura et al. (2022c), Motozono et al. (2021), Saito et al. (2022a), Saito et al. (2022b), Suzuki et al. (2022), and Yamasoba et al. (2022).
Keywords: Cell-based Assays; Microbiology.
【저자키워드】 Microbiology., Cell-based Assays, 【초록키워드】 SARS-CoV-2, coronavirus, Microbiology, protocol, virus, viral entry, variants, Spike protein, Protein, membrane fusion, pathogenicity, reporter, cell types, target cells, acute respiratory syndrome, MONITOR, cell membrane, triggering, living cells, Complete, approach, functional, Suzuki, 【제목키워드】 SARS-CoV-2, monitoring, membrane fusion, cell membrane, living cell,