Abstract
Curcumin, the bioactive compound of the spice Curcuma longa , has already been reported as a potential COVID-19 adjuvant treatment due to its immunomodulatory and anti-inflammatory properties. In this study, SARS-CoV-2 was challenged with curcumin; moreover, curcumin was also coupled with laser light at 445 nm in a photodynamic therapy approach. Curcumin at a concentration of 10 μM, delivered to the virus prior to inoculation on cell culture, inhibited SARS-CoV-2 replication (reduction >99%) in Vero E6 cells, possibly due to disruption of the virion structure, as observed using the RNase protection assay. However, curcumin was not effective as a prophylactic treatment on already-infected Vero E6 cells. Notably, when curcumin was employed as a photosensitizer and blue laser light at 445 nm was delivered to a mix of curcumin/virus prior to the inoculation on the cells, virus inactivation was observed (>99%) using doses of curcumin that were not antiviral by themselves. Photodynamic therapy employing crude curcumin can be suggested as an antiviral option against SARS-CoV-2 infection.
Keywords: SARS-CoV-2; Vero E6 cells; curcumin; in vitro infection; photodynamic therapy.
【저자키워드】 SARS-CoV-2, Curcumin, In vitro infection, VERO E6 cells, photodynamic therapy., 【초록키워드】 COVID-19, Treatment, therapy, Antiviral, SARS-COV-2 infection, in vitro, virus, virus inactivation, cells, Cell culture, immunomodulatory, SARS-CoV-2 replication, photodynamic therapy, RNase, Concentration, dose, Vero E6, reduction, anti-inflammatory properties, virion, prophylactic treatment, approach, effective, reported, inhibited, suggested, Curcuma, 【제목키워드】 optimization, photosensitizer,