Abstract
Discovery of efficacious antiviral agents targeting SARS-CoV-2 main protease (Mpro) is of the highest importance to fight against COVID-19. Here, we describe a simple protocol for high-throughput screening of Mpro inhibitors using a robust fluorescence polarization (FP) assay. Candidate Mpro inhibitors from large compound libraries could be rapidly identified by monitoring the change of millipolarization unit value. This affordable FP assay can be modified to screen antiviral agents targeting virus protease. For complete details on the use and execution of this protocol, please refer to Li et al. (2022), Yan et al. (2021), and Yan et al. (2022c).
Keywords: Biophysics; Cell-based assays; High throughput screening; Protein biochemistry; Protein expression and purification.
【저자키워드】 Biophysics, Cell-based Assays, High Throughput Screening, Protein Biochemistry, Protein expression and purification., 【초록키워드】 COVID-19, protocol, protease, virus, SARS-CoV-2 main protease, MPro, antiviral agent, inhibitor, expression, candidate, purification, Complete, robust, highest, 【제목키워드】 SARS-CoV-2 main protease, inhibitor, robust,