Abstract
The current COVID-19 pandemic caused by SARS-Cov-2 is responsible for more than 6 million deaths globally. The development of broad-spectrum and cost-effective antivirals is urgently needed. Medicinal plants are renowned as a complementary approach in which antiviral natural products have been established as safe and effective drugs. Here, we report that the percolation extract of Spatholobus suberectus Dunn (SSP) is a broad-spectrum viral entry inhibitor against SARS-CoV-1/2 and other enveloped viruses. The viral inhibitory activities of the SSP were evaluated by using pseudotyped SARS-CoV-1 and 2, HIV-1 ADA and HXB2 , and H5N1. SSP effectively inhibited viral entry and with EC 50 values ranging from 3.6 to 5.1 μg/ml. Pre-treatment of pseudovirus or target cells with SSP showed consistent inhibitory activities with the respective EC 50 value of 2.3 or 2.1 μg/ml. SSP blocked both SARS-CoV-2 spike glycoprotein and the host ACE2 receptor. In vivo studies indicated that there was no abnormal toxicity and behavior in long-term SSP treatment. Based on these findings, we concluded that SSP has the potential to be developed as a drug candidate for preventing and treating COVID-19 and other emerging enveloped viruses.
Keywords: H5N1; HIV-1; SARS-CoVs; Spatholobus suberectus Dunn; antivirals.
【저자키워드】 HIV-1, H5N1, SARS-CoVs, antivirals., Spatholobus suberectus Dunn, 【초록키워드】 Treatment, viruses, Antiviral, antivirals, COVID-19 pandemic, ACE2 receptor, Toxicity, viral entry, effective drugs, pseudovirus, SARS-CoV-2 spike glycoprotein, death, plant, inhibitor, complementary, Safe, target cell, treating COVID-19, inhibitory activity, drug candidate, pseudotyped, Host, approach, responsible, blocked, caused, indicated, evaluated, inhibited, ADA, SARS-CoV-1 and 2, 【제목키워드】 SARS-CoV-2, antiviral activity, virus, SARS-CoV-1,