Abstract
Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C 3 N 4 nanosheets and Ru-SiO 2 @folic acid (FA) nanomaterials was designed to realize ultrasensitive detection of SARS-CoV-2 virus (RdRp gene). Firstly, the unique g-C 3 N 4 nanosheets displayed very intense and stable ECL at 460 nm, then the triple helix DNA was stably and vertically bound to g-C 3 N 4 on electrode by high binding affinity between ssDNA and g-C 3 N 4 . Meanwhile, trace amounts of target genes were converted to a large number of output by three-dimensional (3D) DNA walker multiple amplification, and the output bridged a multifunctional probe Ru-SiO 2 @FA to electrode. Ru-SiO 2 @FA not only showed high ECL at 620 nm, but also effectively quenched g-C 3 N 4 ECL. As a result, ECL decreased at 460 nm and increased at 620 nm, which was used to design a rational ECL biosensor for detection of SARS gene. The results show that the biosensor has excellent detection sensitivity for RdRp gene with a dynamic detection range of 1 fM to 10 nM and a limit of detection (LOD) of 0.18 fM. The dual-wavelength ratio ECL biosensor has inestimable value and application prospects in the fields of biosensing and clinical diagnosis.
Keywords: RdRp gene biosensor g-C(3)N(4) nanosheets.
【저자키워드】 RdRp gene biosensor g-C(3)N(4) nanosheets., 【초록키워드】 Treatment, SARS-CoV-2, coronavirus, SARS-CoV-2 virus, binding affinity, amplification, DNA, limit of detection, disease, Clinical diagnosis, Detection sensitivity, ECL, RdRp gene, target gene, energy transfer, syndrome, ssDNA, probe, was used, unique, multifunctional, RET, 【제목키워드】 platform, SARS-CoV-2 RdRp, Electrochemiluminescence, energy transfer,