Abstract
Developing efficient and highly sensitive diagnostic techniques for early detections of pathogenic viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is vitally important for preventing its widespread. However, the conventional polymerase chain reaction (PCR)-based detection features high complexity, excessive time-consumption, and labor-intensiveness, while viral protein-based detections suffer from moderate sensitivity and specificity. Here, a non-PCR but ultrasensitive viral RNA detection strategy is reported based on a facile nanoprobe-coupling strategy without enzymatic amplification, wherein PCR-induced bias and other shortcomings are successfully circumvented. This approach endows the viral RNA detection with ultra-low background to maximum signal ratio in the linear signal amplification by using Au nanoparticles as reporters. The present strategy exhibits 100% specificity toward SARS-CoV-2 N gene, and ultrasensitive detection of as low as 52 cp mL -1 of SARS-CoV-2 N gene without pre-PCR amplification. This approach presents a novel ultrasensitive tool for viral RNA detections for fighting against COVID-19 and other types of pathogenic virus-caused diseases.
Keywords: SARS-CoV-2 N gene; Zn2+ doping; magnetic nanoparticles; nanoprobe-coupling strategy; nucleic acid quick detection.
【저자키워드】 Magnetic nanoparticles, SARS-CoV-2 N gene, nucleic acid quick detection., nanoprobe-coupling strategy, Zn2+ doping, 【초록키워드】 COVID-19, SARS-CoV-2, Diseases, diagnostic, coronavirus 2, amplification, nucleic acid, specificity, Sensitivity and specificity, Viral RNA, respiratory, moderate, pathogenic, SARS-CoV-2 N, widespread, approach, feature, reporters, polymerase chain, reported, linear, Developing, exhibit, pathogenic virus, 【제목키워드】 SARS-CoV-2, detection, RNA,