Abstract
The worldwide spread of coronavirus disease 2019 (COVID-19) highlights the need for rapid, simple, and accurate tests to detect various variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The antigen test, based on the lateral flow immunoassay (LFI), is a suitable “first line of defense” test that enables early identification and timely isolation of patients to minimize viral transmission among communities. However, it is generally less accurate than nucleic acid testing, and its sensitivity needs improvement. Here, a novel rapid detection method is designed to sensitively detect SARS-CoV-2 using isolated gold nanoparticle (AuNP)-assembled SiO 2 core-satellite nanoparticles (SiO 2 @Au CSNPs). Well-grown AuNP satellites in the synthesis of SiO 2 @Au CSNPs significantly enhanced their light absorption, increased the detection sensitivity, and lowered the detection limit by 2 orders of magnitude relative to conventional gold colloids. The proposed system enabled highly sensitive detection of the SARS-CoV-2 nucleocapsid protein with a detection limit of 0.24 pg mL -1 within 20 min. This is the first study to develop a highly sensitive antigen test using the absorption-modulated SiO 2 @Au CSNPs. Our findings demonstrate the capacity of this platform to serve as an effective sensing strategy for managing pandemic conditions and preventing the spread of viral infections.
Keywords: COVID-19; SARS-CoV-2; absorption modulation; core−satellite nanoparticles; high sensitivity; lateral flow immunoassay; nucleocapsid protein.
【저자키워드】 COVID-19, SARS-CoV-2, nucleocapsid protein, lateral flow immunoassay, high sensitivity, core−satellite nanoparticles, absorption modulation, 【초록키워드】 coronavirus disease, coronavirus, pandemic, variant, viral infections, nucleocapsid protein, Antigen, Spread, lateral flow, nucleic acid, sensitivity, Patient, Isolation, Viral transmission, platform, Gold nanoparticle, Detection sensitivity, Detection limit, acute respiratory syndrome, AuNP, effective, highlight, detect, develop, significantly, condition, less, magnitude, colloids, the SARS-CoV-2, 【제목키워드】 detection, nanoparticle, sensitive, Flow, Lateral,