Abstract
Objective: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection.
Results: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing.
Keywords: Ct value; Heat inactivation; RT- PCR; SARS-COV-2.
【저자키워드】 SARS-CoV-2, heat inactivation, Ct value, RT- PCR, 【초록키워드】 Efficacy, diagnostic, virus, SARS-CoV-2 detection, PCR, temperature, molecular, heat-inactivation, Concentration, heat, Effect, heat-inactivated, initial, clinical sample, significantly, changed, 【제목키워드】 reverse transcription PCR, Effect,