Abstract
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS 2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS 2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS 2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.
Keywords: HPV-16; Nanozyme strips; Nucleic acid detection; Point-of-care testing; Recombinase polymerase amplification; SARS-CoV-2.
【저자키워드】 SARS-CoV-2., point-of-care testing, Nucleic acid detection, recombinase polymerase amplification, Nanozyme strips, HPV-16, 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, coronavirus, pandemic, reliability, Diagnosis, RT-PCR, virus, viral infections, point-of-care, amplification, nucleic acid, SARS-CoV-2 detection, limit of detection, POCT, nucleic acid amplification, RPA, acute respiratory syndrome, gold standard, polymerase, REcombinase, clinical sample, develop, subsequent, amplify, unique, catalytic, highlighting, nontoxic, TMB, 【제목키워드】 SARS-CoV-2,