Abstract
The COVID-19 pandemic highlighted the significance of readily available and easily performed viral testing for surveillance during future infectious pandemics. The objectives of this study were: to assess the performance of the Xpert Xpress Flu and/or RSV test, a multiplex PCR assay for detecting influenza A and B virus and respiratory syncytial virus nucleic acids in respiratory tract specimens, relative to the Quidel Lyra Influenza A+B assay and the Prodesse ProFlu+ assay, and the system’s ease of use by minimally trained operators. Overall, the Xpert Xpress Flu/RSV test demonstrated a high positive and negative percent agreement with the comparator assays, and was easy to use and interpret results, based on the operators’ feedback. We concluded that the Xpert Xpress Flu/RSV test is sensitive, specific, and easy to use for the diagnosis of influenza and RSV by minimally trained operators and can be a valuable tool in future infectious clusters or pandemics.
Keywords: CLIA-Waved Environment; Influenza; Multiplex real-time PCR test; RSV.
【저자키워드】 Influenza, RSV., Multiplex real-time PCR test, CLIA-Waved Environment, 【초록키워드】 COVID-19 pandemic, Multiplex PCR, Diagnosis, virus, influenza A, nucleic acid, Surveillance, Real-time PCR, respiratory syncytial virus, RSV, Pandemics, Cluster, positive, operator, operators, respiratory tract specimens, Lyra, performed, assays, demonstrated, 【제목키워드】 Cepheid, operator, performed,