Abstract
Delta (B.1.617.2), a highly infectious variant of SARS-CoV-2, has been sweeping the world, and threatening the safety of human life seriously. It is urgent to develop a highly selective and sensitive assay to accurately identify the SARS-CoV-2 variant Delta. In this work, we constructed a graphene/CRISPR-dCas9 electrochemical biosensor to accurately identify SARS-CoV-2 variant Delta, where the signal was further amplified by embedded electrochemical probe [Ru(phen) 2 dppz]BF 4 . This detection assay could be finished within 47 min totally, with the detection limit of 1.2 pM and good reproducibility with a C·V.% of 2.48% (n = 5). And the biosensor could selectively identify Delta among SARS-CoV-2 and other variants, including Alpha, Beta, Gamma. This assay was further validated by 26 real clinical samples, showing 100% clinical sensitivity and 100% clinical specificity, which provides a new direction for identifying other SARS-CoV-2 variants in the future.
Keywords: Accurate identification; CRISPR-dCas9; Electrochemical biosensor; SARS-CoV-2; Variant delta.
【저자키워드】 SARS-CoV-2, electrochemical biosensor, Variant delta., CRISPR-dCas9, Accurate identification, 【초록키워드】 variant, SARS-CoV-2 variant, Delta, B.1.617.2, clinical samples, Gamma, Beta, Detection limit, clinical sensitivity, other variants, clinical specificity, reproducibility, selective, probe, variant of SARS-CoV-2, amplified, identify, develop, provide, the SARS-CoV-2, 【제목키워드】 SARS-CoV-2 variant,