Abstract
The main protease (M pro ), which is highly conserved and plays a critical role in the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a natural biomarker for SARS-CoV-2. Accurate assessment of the M pro activity is crucial for the detection of SARS-CoV-2. Herein, we report a nanopore-based sensing strategy that uses an enzyme-catalyzed cleavage reaction of a peptide substrate to measure the M pro activity. The peptide was specifically cleaved by the M pro , thereby releasing the output products that, when translocated through aerolysin, quantitatively produced the signature current events. The proposed method exhibited high sensitivity, allowing the detection of M pro concentrations as low as 1 nM without the use of any signal amplification techniques. This simple, convenient, and label-free nanopore assay may expand the diagnostic tools for viruses.
Keywords: Mpro; SARS-CoV-2; enzyme; nanopore; sensors.
【저자키워드】 SARS-CoV-2, Nanopore, MPro, enzyme, sensors., 【초록키워드】 viruses, coronavirus, Biomarker, diagnostic, peptide, protease, Replication, amplification, sensitivity, cleavage, Critical, Concentration, acute respiratory syndrome, M pro, produced, conserved, events, exhibited, expand, cleaved, translocated, 【제목키워드】 activity,