Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 × 10 × 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35 th cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003). Designing efficient, rapid and low-cost diagnostic technologies targeting nucleic acids remains a challenge. Here the authors present a disposable silicon-based integrated Point-of-Need transducer produced in a standard wet lab and able to chemically-amplify and detect pathogen-specific sequences of nucleic acids quantitatively in real-time.
【저자키워드】 Electrical and electronic engineering, Sensors and biosensors, Sensors and probes, 【초록키워드】 Treatment, SARS-CoV-2, diagnostic, Diagnosis, Intervention, Symptom, Novel coronavirus, Laboratory, nucleic acid, specificity, infections, pathogen, Real-time PCR, nucleic acids, Rapid, Pathogens, Quantitative, Device, cDNA, fragment of, agent, silicon, novel coronavirus SARS-CoV-2, sequence, Genomic DNA, Mycobacterium, lab, effective, Course, produced, detect, against SARS-CoV, pathogen-specific, disease-causing,