Abstract
With rapid growing of environmental contact infection, more and more attentions are focused on the precise and absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus on cold chain foods via point-of-care test (POCT). In this work, we propose a hydrogel-mediated reverse transcription loop-mediated isothermal amplification (RT-LAMP) for ultrafast and absolute quantification of SARS-CoV-2. Cross-linked hydrogel offers opportunities for digital single molecule amplification in nanoconfined spaces, facilitating the virus lysis, RNA reverse transcription and amplification process, which is about 3.4-fold faster than conventional bulk RT-LAMP. Ultrafast quantification of SARS-CoV-2 is accomplished in 15 min without virus pre-lysis and RNA extraction. The sensitivity can accurately quantify SARS-CoV-2 down to 0.5 copy/μL. Furthermore, the integrated system has an excellent specificity, reproducibility and storage stability, which can be also used to test SARS-CoV-2 on various cold chain fruits. The developed ultrafast and simple hydrogel RT-LAMP will be an enormous potential for surveillance of virus or other hazardous microbes in environmental, agricultural and food industry.
Keywords: Absolute quantification; Cold chain food; Hydrogel RT-LAMP; SARS-CoV-2; Ultrafast detection.
【저자키워드】 SARS-CoV-2, Absolute quantification, Cold chain food, Ultrafast detection., Hydrogel RT-LAMP, 【초록키워드】 coronavirus, virus, RNA, RNA extraction, point-of-care, amplification, RT-LAMP, sensitivity, specificity, stability, Surveillance, POCT, reverse transcription, isothermal amplification, hydrogel, cold chain, quantification, Food industry, microbe, acute respiratory syndrome, reproducibility, offer, faster, contact infection, 【제목키워드】 RT-LAMP, quantification,