Abstract
mRNA vaccines for SARS-CoV-2 have been widely used and saving millions of people in the world. How efficiently proteins are produced from exogenous mRNAs in the embryonic brain, however, is less known. Here we show that protein expression occurs highly efficiently in neural stem cells, in a very narrow time window after mRNA electroporation in the embryonic mouse brain, where plasmids have been successfully transfected. Protein expression is detected 1 h and 12 h after the electroporation of mRNAs and plasmids, respectively. The delivery of exogenous mRNAs may be useful for not only vaccines but also functional analysis in the brain.
All Keywords
【초록키워드】 stem cells, SARS-CoV-2, Vaccine, Brain, Protein, mRNA, expression, Analysis, Electroporation, Plasmid, protein expression, plasmids, produced, functional, less, occur, embryonic, 【제목키워드】 Brain, mRNA, protein expression, embryonic, Immediate,
【초록키워드】 stem cells, SARS-CoV-2, Vaccine, Brain, Protein, mRNA, expression, Analysis, Electroporation, Plasmid, protein expression, plasmids, produced, functional, less, occur, embryonic, 【제목키워드】 Brain, mRNA, protein expression, embryonic, Immediate,