Transfer of convalescent plasma (CP) had been proposed early during the SARS-CoV-2 pandemic as an accessible therapy, yet trial results worldwide have been mixed, potentially due to the heterogeneous nature of CP. Here we perform deep profiling of SARS-CoV-2-specific antibody titer, Fc-receptor binding, and Fc-mediated functional assays in CP units, as well as in plasma from hospitalized COVID-19 patients before and after CP administration. The profiling results show that, although all recipients exhibit expanded SARS-CoV-2-specific humoral immune responses, CP units contain more functional antibodies than recipient plasma. Meanwhile, CP functional profiles influence the evolution of recipient humoral immunity in conjuncture with the recipient’s pre-existing SARS-CoV2-specific antibody titers: CP-derived SARS-CoV-2 nucleocapsid-specific antibody functions are associated with muted humoral immune evolution in patients with high titer anti-spike IgG. Our data thus provide insights into the unexpected impact of CP-derived functional anti-spike and anti-nucleocapsid antibodies on the evolution of SARS-CoV-2-specific response following severe infection. Convalescent plasma (CP) has been trialed as a therapy for SARS-CoV-2 symptoms, but its heterogenous nature precludes uniform outcomes. Here the authors perform deep profiling of CP, as well as plasma of CP recipients before and after transfer, to find CP-mediated, spike/nucleocapsid-focused modulations of humoral responses in the recipient.
【저자키워드】 antibodies, SARS-CoV-2, viral infection, Translational immunology, 【초록키워드】 convalescent plasma, Evolution, Severe infection, pandemic, therapy, Trial, antibody, SARS-CoV-2 pandemic, Symptoms, immune, outcomes, Humoral immunity, Humoral response, anti-Spike IgG, immune responses, Patient, Antibody titer, plasma, humoral responses, humoral immune responses, function, Anti-spike, binding, Anti-nucleocapsid antibodies, hospitalized COVID-19 patient, administration, anti-nucleocapsid antibody, Receptor binding, humoral, hospitalized COVID-19 patients, modulation, profile, transfer, SARS-CoV-2 nucleocapsid, recipient, heterogenous, heterogeneous nature, nucleocapsid-specific antibody, functional, the SARS-CoV-2, 【제목키워드】 immune response, severe COVID-19, antibody,