COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome. Here, Kim et al. apply various sequencing techniques (RPF-seq, QTI-seq, mRNA-seq, sRNA-seq) to unravel the high-resolution, longitudinal translatome and transcriptome of SARS-CoV-2. They identify a translation initiation site in the leader sequence of all genomic and subgenomic RNAs and show its relevance for the SARS-CoV-2 translatome.
【저자키워드】 SARS-CoV-2, translation, 【초록키워드】 COVID-19, Transcriptome, coronavirus, Sequencing, severe acute respiratory syndrome Coronavirus, mRNA, subgenomic RNA, leader sequence, respiratory, genomic, function, ORFs, Human genome, deaths, High-resolution, acute respiratory syndrome, Regulation, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, human cells, help, subgenomic RNAs, enhancer, sRNA, suppressor, downstream, translation initiation, infecting, resulting, identify, evaluate, caused, ORF, provide, unique, regulated, initiate, the SARS-CoV-2, 【제목키워드】 High-resolution, the SARS-CoV-2,