Abstract
Background: SARS-CoV-2 is one of the most contagious viruses in the Coronaviridae (CoV) family, which has become a pandemic. The aim of this study is to understand more about the role of hsa_circ_0004812 in the SARS-CoV-2 related cytokine storm and its associated molecular mechanisms.
Materials and methods: cDNA synthesis was performed after total RNA was extracted from the peripheral blood mononuclear cells (PBMC) of 46 patients with symptomatic COVID-19, 46 patients with asymptomatic COVID-19, and 46 healthy controls. The expression levels of hsa_circ_0004812, hsa-miR-1287-5p, IL6R, and RIG-I were determined using qRT-PCR, and the potential interaction between these molecules was confirmed by bioinformatics tools and correlation analysis.
Results: hsa_circ_0004812, IL6R, and RIG-I are expressed higher in the severe symptom group compared with the negative control group. Also, the relative expression of these genes in the asymptomatic group is lower than in the severe symptom group. The expression level of hsa-miR-1287-5p was positively correlated with symptoms in patients. The results of the bioinformatics analysis predicted the sponging effect of hsa_circ_0004812 as a competing endogenous RNA on hsa-miR-1287-5p. Moreover, there was a significant positive correlation between hsa_circ_0004812, RIG-I, and IL-6R expressions, and also a negative expression correlation between hsa_circ_0004812 and hsa-miR-1287-5p and between hsa-miR-1287-5p, RIG-I, and IL-6R.
Conclusion: The results of this in-vitro and in silico study show that hsa_circ_0004812/hsa-miR-1287-5p/IL6R, RIG-I can play an important role in the outcome of COVID-19.
Keywords: SARS-CoV-2; cytokine storm; hsa-miR-1287-5p; hsa_circ_0004812; sponging effect.
【저자키워드】 SARS-CoV-2, Cytokine storm, sponging effect., hsa_circ_0004812, hsa-miR-1287-5p, 【초록키워드】 COVID-19, pandemic, qRT-PCR, bioinformatics, cytokine, Symptom, in silico, outcome, RNA, Peripheral blood, Asymptomatic, symptomatic, Patient, CoV, RIG-I, PBMC, correlation, Bioinformatics analysis, expression, patients, Interaction, In-vitro, mononuclear cell, Correlation analysis, expression level, relative expression, positive correlation, molecular mechanisms, IL-6R, healthy controls, material, IL6R, total RNA, contagious, expressions, negative control, cDNA synthesis, predicted, virus, was performed, expressed, competing, the Coronaviridae, patients with asymptomatic, positively correlated, the SARS-CoV-2, 【제목키워드】 RIG-I, COVID-19 cytokine storm, promote,