Abstract
In the present manuscript we analyzed the influence of hypoxic response in Caco-2 cells on the expression of genes and miRNAs involved in the mechanisms of intracellular transport of SARS-CoV-2 viral particles, especially endocytosis and transcytosis. With the use of RNA sequencing of Caco-2 cells treated with hypoxia-inducing oxyquinoline derivative, we showed two-fold increase in the expression of the main SARS-CoV-2 receptor ACE2. Expression of the non-canonical receptor TFRC was also elevated. We also observed a significant increase in the expression levels of genes from the low-density lipoprotein (LDL) receptor family, which play a crucial role in the transcytosis: LDLR, LRP1, LRP4, and LRP5. Upregulation of LDLR was coupled with the downregulation of hsa-miR-148a-3p, which can directly bind to LDLR mRNA. Thus, the hypoxic response in Caco-2 cells includes upregulation of genes involved in the mechanisms of endocytosis and transcytosis of SARS-CoV-2 viral particles.
Keywords: ACE2; Caco-2; LDL; SARS-CoV-2; hypoxia; intestine; miRNA; oxyquinoline; transcriptome.
【저자키워드】 SARS-CoV-2, ACE2, hypoxia, miRNA, intestine, Transcriptome., LDL, Caco-2, oxyquinoline, 【초록키워드】 Transcriptome, SARS-CoV-2 receptor, mRNA, RNA sequencing, receptor, expression, mechanism, Transport, expression level, significant increase, upregulation, low-density, downregulation, manuscript, LDLR, SARS-CoV-2 viral particles, hypoxic, analyzed, include, involved, elevated, treated, increase in, expression of gene, Caco-2 cell, LRP1, LRP4, LRP5, TFRC, 【제목키워드】 endocytosis, response, induction, transcytosis,