Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that infectious virus shedding is detected by virus cultures in 23 of the 129 patients (17.8%) hospitalized with COVID-19. The median duration of shedding infectious virus is 8 days post onset of symptoms (IQR 5–11) and drops below 5% after 15.2 days post onset of symptoms (95% confidence interval (CI) 13.4–17.2). Multivariate analyses identify viral loads above 7 log 10 RNA copies/mL (odds ratio [OR] of 14.7 (CI 3.57-58.1; p < 0.001) as independently associated with isolation of infectious SARS-CoV-2 from the respiratory tract. A serum neutralizing antibody titre of at least 1:20 (OR of 0.01 (CI 0.003-0.08; p < 0.001) is independently associated with non-infectious SARS-CoV-2. We conclude that quantitative viral RNA load assays and serological assays could be used in test-based strategies to discontinue or de-escalate infection prevention and control precautions. Duration of infectious SARS-CoV-2 shedding is an important measure for improved disease control. Here, the authors use virus cultures of respiratory tract samples from COVID-19 patients and observe a median shedding duration of 8 days and a drop below 5% after 15,2 days post onset of symptoms.
【저자키워드】 SARS-CoV-2, Translational research, viral infection, 【초록키워드】 COVID-19, Hospitalized, virus, RNA, Duration, Viral, Serological assay, Culture, Viral load, serological assays, RNA copies, disease control, Patient, Isolation, Infection prevention, respiratory tract, determinants, respiratory, viral RNA load, disease, Quantitative, COVID-19 patients, Prevention and control, Infectious virus, Analysis, Odds ratio, COVID-19 patient, virus cultures, determinant, 95% confidence interval, viral loads, multivariate, titre, drop, multivariate analyses, non-infectious, Serum neutralizing antibody, respiratory tract samples, precautions, observé, log, identify, median, onset of symptom, question, onset of symptoms, respiratory tract sample, IQR, with COVID-19, 【제목키워드】 COVID-19, coronavirus, Duration, determinant, hospitalized patient,