The COVID-19 pandemic affects more than 81 million people worldwide with over 1.7 million deaths. As the population returns to work, it is critical to develop tests that reliably detect SARS-CoV-2-specific antibodies. Here we present results from a multiplex serology test for assessing the antibody responses to COVID-19. In an initial large cohort, this test shows greater than 99% agreement with COVID-19 PCR test. In a second outpatient cohort consisting of adults and children in Colorado, the IgG responses are more robust in positive/symptomatic participants than in positive/asymptomatic participants, the IgM responses in symptomatic participants are transient and largely fall below the detection limit 30 days after symptom onset, and the levels of IgA against SARS-CoV-2 receptor binding domain are significantly increased in participants with moderate-to-severe symptoms compared to those with mild-to-moderate symptoms or asymptomatic individuals. Our results thus provide insight into serology profiling and the immune response to COVID-19. Antibody responses to SARS-CoV-2 may be important biomarkers for assessing the risk for viral transmission. Here the authors present serological antibody profiling results of COVID-19 patients using a new multiplex assay to show distinct kinetics and dynamics of IgG, IgM and IgA responses in patients with different disease severity.
【저자키워드】 antibodies, viral infection, Diagnostic markers, Antimicrobial responses, 【초록키워드】 COVID-19, SARS-CoV-2, IgG, IgM, immune response, Biomarker, serology, children, COVID-19 pandemic, disease severity, Antibody Response, risk, Symptom, SARS-CoV-2 receptor, Cohort, Antibody responses, Viral, Multiplex assay, Asymptomatic individuals, multiplex serology, IgA, Patient, Viral transmission, large cohort, PCR test, serological, Critical, SARS-CoV-2-specific antibodies, COVID-19 patients, antibody profiling, Detection limit, deaths, COVID-19 patient, Mild-to-moderate, IgG responses, IgG response, symptom onset, Participants, IgM responses, participant, symptomatic participants, binding domain, IgA responses, IgM response, COVID-19 PCR test, IgA response, Affect, robust, initial, significantly increased, greater, detect, develop, the antibody response, symptomatic participant, with COVID-19, 【제목키워드】 Patient, chemiluminescent immunoassay, multiplex, serological, symptomatic and asymptomatic,