Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. Current SARS-CoV-2 diagnostic methods are sensitive yet poorly suited to testing whole communities on a regular basis. Here the authors present Cap-iLAMP that tests gargle lavage samples with an improved colorimetric RT-LAMP.
【저자키워드】 viral infection, Diagnostic markers, Assay systems, 【초록키워드】 SARS-CoV-2, protocol, equipment, Automation, Laboratory, Spread, RNA extraction, point-of-care, RT-LAMP, Viral, Loop-mediated isothermal amplification, nucleic acids, LAMP, Smartphone, Community, isothermal amplification, diagnostic methods, False positive, Diagnostic method, False positives, capture, Euro, individual, viral nucleic acid, viral nucleic acids, positive sample, Prevent, current, tested, addition, less, reducing, 【제목키워드】 SARS-CoV-2, capture,