SARS-CoV-2 infection has been shown to trigger a wide spectrum of immune responses and clinical manifestations in human hosts. Here, we sought to elucidate novel aspects of the host response to SARS-CoV-2 infection through RNA sequencing of peripheral blood samples from 46 subjects with COVID-19 and directly comparing them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS-CoV-2 infection triggers a powerful transcriptomic response in peripheral blood with conserved components that are heavily interferon-driven but also marked by indicators of early B-cell activation and antibody production. Interferon responses during SARS-CoV-2 infection demonstrate unique patterns of dysregulated expression compared to other infectious and healthy states. Heterogeneous activation of coagulation and fibrinolytic pathways are present in early COVID-19, as are IL1 and JAK/STAT signaling pathways, which persist into late disease. Classifiers based on differentially expressed genes accurately distinguished SARS-CoV-2 infection from other acute illnesses (auROC 0.95 [95% CI 0.92–0.98]). The transcriptome in peripheral blood reveals both diverse and conserved components of the immune response in COVID-19 and provides for potential biomarker-based approaches to diagnosis. The systemic immune features that distinguish COVID-19 from common infections remain incompletely elucidated. Here McClain et al. compare RNA sequencing in peripheral blood between subjects with SARS-CoV-2 and other respiratory infections and demonstrate dysregulated immune responses in COVID-19 with both heterogeneous and conserved components.
【저자키워드】 SARS-CoV-2, viral infection, Infectious-disease diagnostics, 【초록키워드】 COVID-19, respiratory infections, Transcriptome, coronavirus, immune response, Pneumonia, Influenza, SARS-COV-2 infection, Sequencing, Infection, interferon, Diagnosis, host response, immune, Coagulation, Peripheral blood, clinical manifestations, immune responses, response, RNA sequencing, pathway, respiratory, bacterial pneumonia, disease, expression, Differentially expressed genes, differentially expressed gene, B-cell, Bacterial, STAT, Pathways, Trigger, antibody production, triggers, clinical manifestation, Activation, subject, components, other respiratory infections, fibrinolytic, hosts, healthy controls, dysregulated immune response, heterogeneous, AUROC, peripheral blood samples, component, dysregulated immune responses, classifiers, transcriptomic, approach, feature, JAK/STAT signaling, shown, conserved, healthy, provide, unique, other respiratory infection, reveal, dysregulated, illness, with COVID-19, 【제목키워드】 transcriptional response,