Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of the purified SARS-CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor detects these important antibodies. ABSTRACT Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of the purified SARS-CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor detects these important antibodies. The cPass surrogate virus neutralization test (sVNT), compared directly with eight SARS-CoV-2 IgG serology and two live-cell neutralization tests, gives similar or improved accuracy for qualitative delineation between positive and negative individuals in a fast, scalable, and high-throughput assay. The combined data support the cPass sVNT as a tool for highly accurate SARS-CoV-2 immunity surveillance of infected/recovered and/or vaccinated individuals as well as drug and convalescent-phase donor screening. The data also preview a novel application for the cPass sVNT in calibrating the stringency of live-cell neutralization tests and its use in longitudinal testing of recovered and/or vaccinated patients.
【저자키워드】 SARS-CoV-2, Vaccines, Neutralizing antibodies, serology, ELISA, 【초록키워드】 antibodies, coronavirus, antibody, SARS-CoV-2 immunity, Spike protein, hACE2, Receptor binding domain, Reinfection, Surveillance, Accuracy, RBD, serology tests, virus neutralization test, SARS-CoV-2 spike protein, neutralization test, serology test, Neutralizing, receptor, respiratory, patients, SARS-CoV-2 IgG, Donor, Interaction, angiotensin, neutralization tests, Support, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, individual, sVNT, SARS-CoV-2 spike protein receptor binding domain, domain, human Angiotensin-converting enzyme, vaccinated individuals, positive, vaccinated individual, Extracellular, detect, eight, less, expected, purified, 【제목키워드】 detection, response, tracing, New,