Management of the coronavirus disease 2019 (COVID-19) pandemic requires widespread testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A main limitation for widespread SARS-CoV-2 testing is the global shortage of essential supplies, among them RNA extraction kits. ABSTRACT Management of the coronavirus disease 2019 (COVID-19) pandemic requires widespread testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A main limitation for widespread SARS-CoV-2 testing is the global shortage of essential supplies, among them RNA extraction kits. The need for commercial RNA extraction kits places a bottleneck on tests that detect SARS-CoV-2 genetic material, including PCR-based reference tests. Here, we propose an alternative method we call PEARL ( p recipitation- e nhanced a nalyte r etrieva l ) that addresses this limitation. PEARL uses a lysis solution that disrupts cell membranes and viral envelopes while simultaneously providing conditions suitable for alcohol-based precipitation of RNA, DNA, and proteins. PEARL is a fast, low-cost, and simple method that uses common laboratory reagents and offers performance comparable to that of commercial RNA extraction kits. PEARL offers an alternative method to isolate host and pathogen nucleic acids and proteins to streamline the detection of DNA and RNA viruses, including SARS-CoV-2.
【저자키워드】 SARS-CoV-2, virus detection, field deployable, rapid isolation of RNA, DNA and protein, 【초록키워드】 COVID-19, coronavirus disease, viruses, Coronavirus disease 2019, coronavirus, pandemic, Proteins, alcohol, severe acute respiratory syndrome Coronavirus, Laboratory, RNA, Protein, nucleic acid, DNA, Viral, pathogen, SARS-CoV-2 testing, RNA viruses, respiratory, Streamline, alternative method, reference tests, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, RNA extraction kits, cell membranes, reagent, genetic material, offer, cell membrane, viral envelope, PEARL, Host, widespread, DNA and RNA, detect, condition, comparable, disrupt, RNA extraction kit, 【제목키워드】 detection, RNA, DNA,