Highlights • Both assays discriminate SARS-CoV-2 seropositive and seronegative individuals. • Both assays correctly estimate seroprevalence in a wide range of seroprevalences. • WANTAI has slightly higher sensitivity than KWTRP. • KWTRP has slightly higher specificity than WANTAI. Many SARS-CoV-2 antibody detection assays have been developed but their differential performance is not well described. In this study we compared an in-house (KWTRP) ELISA which has been used extensively to estimate seroprevalence in the Kenyan population with WANTAI, an ELISA which has been approved for widespread use by the WHO. Using a wide variety of sample sets including pre-pandemic samples (negative gold standard), SARS-CoV-2 PCR positive samples (positive gold standard) and COVID-19 test samples from different periods (unknowns), we compared performance characteristics of the two assays. The overall concordance between WANTAI and KWTRP was 0.97 (95% CI, 0.95–0.98). For WANTAI and KWTRP, sensitivity was 0.95 (95% CI 0.90–0.98) and 0.93 (95% CI 0.87–0.96), respectively. Specificity for WANTAI was 0.98 (95% CI, 0.96–0.99) and 0.99 (95% CI 0.96–1.00) while KWTRP specificity was 0.99 (95% CI, 0.98–1.00) and 1.00 using pre-pandemic blood donors and pre-pandemic malaria cross-sectional survey samples respectively. Both assays show excellent characteristics to detect SARS-CoV-2 antibodies.
【저자키워드】 SARS-CoV-2, IgG, serology, immunoassay, Total immunoglobulin, 【초록키워드】 Seroprevalence, ELISA, malaria, sensitivity, specificity, Blood donors, SARS-CoV-2 antibodies, Characteristics, SARS-CoV-2 antibody, gold, seropositive, seronegative, SARS-CoV-2 PCR, COVID-19 test, cross-sectional survey, blood donor, 95% CI, house, gold standard, positive, positive sample, overall concordance, widespread, described, detect, assays, approved, variety, the WHO, individuals, 【제목키워드】 Population, Spike protein, Protein, SARS-CoV-2 antibody, Immunoglobulin, Receptor binding, Comparative,