Since severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is producing a large number of infections and deaths globally, the development of supportive and auxiliary treatments is attracting increasing attention. Here, we evaluated SARS-CoV-2-inactivation activity of the polyphenol-rich tea leaf extract TY-1 containing concentrated theaflavins and other virucidal catechins. The TY-1 was mixed with SARS-CoV-2 solution, and its virucidal activity was evaluated. To evaluate the inhibition activity of TY-1 in SARS-CoV-2 infection, TY-1 was co-added with SARS-CoV-2 into cell culture media. After 1 h of incubation, the cell culture medium was replaced, and the cells were further incubated in the absence of TY-1. The viral titers were then evaluated. To evaluate the impacts of TY-1 on viral proteins and genome, TY-1-treated SARS-CoV-2 structural proteins and viral RNA were analyzed using western blotting and real-time RT-PCR, respectively. TY-1 showed time- and concentration-dependent virucidal activity. TY-1 inhibited SARS-CoV-2 infection of cells. The results of western blotting and real-time RT-PCR suggested that TY-1 induced structural change in the S2 subunit of the S protein and viral genome destruction, respectively. Our findings provided basic insights in vitro into the possible value of TY-1 as a virucidal agent, which could enhance the current SARS-CoV-2 control measures.
【저자키워드】 SARS-CoV-2, catechin, polyphenol, tea leaf extract, theaflavin, virucidal activity, 【초록키워드】 Treatment, coronavirus, S protein, SARS-COV-2 infection, Genome, Infection, media, Viral proteins, in vitro, severe acute respiratory syndrome Coronavirus, RT-PCR, Viral, Measures, cells, Cell culture, Impact, virucidal, death, structural proteins, Viral RNA, structural protein, culture medium, respiratory, real-time RT-PCR, polyphenol, Incubation, S2 subunit, viral genome, the cell, the cells, culture media, acute respiratory syndrome, Viral protein, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, viral titers, western blotting, Ty-1, viral titer, cell culture medium, inhibition activity, Cell, ENhance, analyzed, evaluate, evaluated, inhibited, provided, absence, suggested, replaced, the S protein, producing, incubated, auxiliary, 【제목키워드】 activity, Other, Extract, leaf, tea,