ABSTRACT Mounting evidence indicates that SARS-CoV-2 can infect multiple systemic tissues, but few studies have evaluated SARS-CoV-2 RNA dynamics in multiple specimen types due to their reduced accessibility and diminished performance of RT-qPCR with non-respiratory specimens. Here, we employed an ultrasensitive CRISPR-RT–PCR assay to analyze longitudinal mucosal (nasal, buccal, pharyngeal, and rectal), plasma, and breath samples from SARS-CoV-2-infected non-human primates (NHPs) to detect dynamic changes in SARS-CoV-2 RNA level and distribution among these specimens. We observed that CRISPR-RT–PCR results consistently detected SARS-CoV-2 RNA in all sample types at most time points post-infection, and that SARS-CoV-2 infection dose and administration route did not markedly affect the CRISPR-RT–PCR signal detected in most specimen types. However, consistent RT-qPCR positive results were restricted to nasal, pharyngeal, and rectal swab samples, and tended to decrease earlier than CRISPR-RT–PCR results, reflecting lower assay sensitivity. SARS-CoV-2 RNA was detectable in both pulmonary and extrapulmonary specimens from early to late infection by CRISPR-RT–PCR, albeit with different abundance and kinetics, with SARS-CoV-2 RNA increases detected in plasma and rectal samples trailing those detected in upper respiratory tract samples. CRISPR-RT–PCR assays for SARS-CoV-2 RNA in non-respiratory specimens may thus permit direct diagnosis of suspected COVID-19 cases missed by RT–PCR, while tracking SARS-CoV-2 RNA in minimally invasive alternate specimens may better evaluate the progression and resolution of SARS-CoV-2 infections.
【저자키워드】 SARS-CoV-2, CRISPR, RT-qPCR, non-human primate, multi-site specimens, 【초록키워드】 SARS-COV-2 infection, Infection, Diagnosis, nasal, progression, CRISPR, sensitivity, RT-qPCR, Kinetics, NHPs, breath, plasma, SARS-CoV-2 RNA, respiratory, distribution, upper respiratory tract, specimens, SARS-CoV-2 infections, Evidence, mucosal, administration, dose, Post-infection, tissues, COVID-19 case, positive result, specimen, pharyngeal, infect, rectal, RT–PCR, Affect, rectal swab, decrease, invasive, detect, evaluate, detectable, evaluated, reduced, indicate, increase, changes in, Mounting, 【제목키워드】 non-human primate model, change, reveal,