Development of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG antibodies at titers more than 1:100,000 were detectable in all PCR+ subjects ( n = 115) and were absent in the negative controls. Other isotype antibodies (IgA, IgG1-4) were also detected. SARS-CoV-2 neutralization was determined in COVID-19 and convalescent plasma at up to 10,000-fold dilution, using Spike protein pseudotyped lentiviruses, which were also blocked by neutralizing antibodies (NAbs). Hospitalized patients had up to 3000-fold higher antibody and neutralization titers compared to outpatients or convalescent plasma donors. Interestingly, some COVID-19 patients also possessed NAbs against SARS-CoV Spike protein pseudovirus. Together these results demonstrate the high specificity and sensitivity of our assays, which may impact understanding the quality or duration of the antibody response during COVID-19 and in determining the effectiveness of potential vaccines. Using SARS-CoV-2-specific antibody- and neutralization assays, Dogan et al find that hospitalized individuals show higher IgG antibody responses and higher neutralization titers compared to outpatient or convalescent plasma donors, providing insights into the host humoral response to SARS CoV-2.
【저자키워드】 antibodies, SARS-CoV-2, Translational research, Infectious-disease diagnostics, 【초록키워드】 COVID-19, SARS CoV-2, convalescent plasma, neutralizing antibody, public health, Vaccine development, Hospitalized, Vaccines, Neutralizing antibodies, spike, antibody, SARS-COV-2 infection, Antibody Response, hospitalized patients, Spike protein, nucleocapsid protein, Protein, sensitivity, specificity, IgG antibody, IgG antibodies, pseudovirus, IgA, response, SARS-CoV-2 spike protein, Patient, Effectiveness, outpatients, donors, development, Other, IgG1, SARS-CoV-2 neutralization, NAb, neutralization titers, Outpatient, COVID-19 patient, titers, humoral, Dilution, negative controls, subject, SARS CoV, individual, NAbs, SARS-CoV spike protein, pseudotyped lentiviruses, neutralization titer, neutralization assays, Host, blocked, assays, detectable, was determined, question, against SARS-CoV, the antibody response, specific IgG antibody, 【제목키워드】 antibody, virus, Neutralization assay, humoral immune response,