There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. The combination of three viral antigens allows for the interrogation of full seroconversion. Importantly, we have detected N-reactive antibodies in COVID-19-negative individuals. Here we present an immunoassay that can be easily implemented and has superior potential to detect low antibody titers compared to current gold standard serology methods. Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.
【저자키워드】 antibodies, viral infection, 【초록키워드】 SARS-CoV-2, pandemic, serology, antibody, ELISA, Spike protein, Laboratory, Protein, immunoassay, Cohort, Seroconversion, Viral, IgM antibodies, RBD, Antibody titer, gold, spike receptor-binding domain, nucleoprotein, ELISAs, Antibody titers, Antibody titre, Combination, SARS-CoV-2 proteins, IgG and IgM, Viral antigen, subunit, viral antigens, gold standard, individual, immunogenic, reactive, flow cytometric, performed, detect, collected, develop, the spike protein, individuals, 【제목키워드】 SARS-CoV-2, antibody, flow cytometry, sensitive, individual, reveal,