Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2–positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April–May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.
【저자키워드】 COVID-19, coronavirus disease, severe acute respiratory syndrome coronavirus 2, viruses, respiratory infections, zoonoses, SARS-CoV-2, Coronaviruses, ELISA, nucleocapsid protein, Receptor-binding domain, cats, dogs, Virus neutralization, The Netherlands, serologic screening, 【초록키워드】 coronavirus disease, severe acute respiratory syndrome coronavirus 2, coronavirus, Stress, SARS-COV-2 infection, Seroprevalence, severe acute respiratory syndrome Coronavirus, ELISA, Antigen, Protein, Antibody detection, cats, animals, Virus neutralization, respiratory, serologic assays, SARS-CoV-2 infections, seropositive, Netherlands, acute respiratory syndrome, acute respiratory syndrome coronavirus, serum samples, SARS-CoV-2 nucleocapsid, serum sample, infect, analyzed, Netherland, SARS-CoV-2–positive, 【제목키워드】 coronavirus, coronavirus 2, Screening, dog, serologic, respiratory, Wave, Netherland,