Abstract Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.
All Keywords
【저자키워드】 SARS-CoV-2, RT-qPCR, Contamination, Molecular diagnosis, False positive, 【초록키워드】 COVID-19, coronavirus, Transcription, nucleic acid, Viral, Pathogens, cross, common cold, RT-PCR assay, Diagnostic method, analytical sensitivity, viral genome, RT-PCR assays, viral pathogens, selected, detect, applied, nucleic acid target, 【제목키워드】 molecular, reagent,
【저자키워드】 SARS-CoV-2, RT-qPCR, Contamination, Molecular diagnosis, False positive, 【초록키워드】 COVID-19, coronavirus, Transcription, nucleic acid, Viral, Pathogens, cross, common cold, RT-PCR assay, Diagnostic method, analytical sensitivity, viral genome, RT-PCR assays, viral pathogens, selected, detect, applied, nucleic acid target, 【제목키워드】 molecular, reagent,
초 록 전 세계적으로 코로나19와의 싸움에서 적용되고 있는 주요 진단법인 역전사(RT)-PCR은 바이러스 게놈의 단일 분자를 검출할 수 있다. SARS-CoV-2에 대해 올바르게 설계되고 실행된 RT-PCR 분석은 유사하지만 구별되는 바이러스 병원체(예: 감기와 관련된 코로나바이러스)와 교차 반응하지 않아야 하며 매우 높은 분석 감도로 수행해야 합니다. 이 분석 성능은 위양성 신호의 검출 없이 선택된 핵산 표적의 존재를 검출하는 방법의 능력에 근거합니다.