In the context of an unprecedented shortage of nasopharyngeal swabs (NPS) or sample transport media during the coronavirus disease 2019 (COVID-19) crisis, alternative methods for sample collection are needed. To address this need, we validated a cell culture medium as a viral transport medium, and compared the analytical sensitivity of SARS-CoV-2 RT-PCR in nasal wash (NW), oropharyngeal swab (OPS), and NPS specimens. Both the clinical and analytical sensitivity were comparable in these three sample types. OPS and NW specimens may therefore represent suitable alternatives to NPS for SARS-CoV-2 detection. Electronic supplementary material The online version of this article (10.1007/s10096-020-04039-8) contains supplementary material, which is available to authorized users.
【저자키워드】 SARS-CoV-2, upper respiratory tract infection, Nasopharyngeal swab, oropharyngeal swab, Diagnostic techniques, Nasal washes, 【초록키워드】 COVID-19, coronavirus disease, Coronavirus disease 2019, nasal, SARS-CoV-2 detection, Viral, Transport media, nasopharyngeal swabs, oropharyngeal swab, Cell culture, culture medium, SARS-CoV-2 RT-PCR, crisis, specimens, analytical sensitivity, alternative methods, alternative method, transport medium, Viral transport medium, supplementary material, specimen, sample collection, cell culture medium, NPs, comparable, 【제목키워드】 nasal, nasopharyngeal, Oropharyngeal, specimen,