Background The outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study. Methods RT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated. Results The performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL. Conclusion A quantitative detection of the COVID-19 virus based on RT-PCR was established. Supplementary Information The online version contains supplementary material available at 10.1186/s40001-021-00608-5.
【저자키워드】 COVID-19, SARS-CoV-2 virus, RT-PCR, RNA, Quantitative, 【초록키워드】 coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, Efficacy, Vaccine, coronavirus, antiviral drugs, SARS-CoV-2 virus, novel coronavirus disease, severe acute respiratory syndrome Coronavirus, RT-PCR, virus, antiviral drug, Novel coronavirus, Viral, Viral load, outbreak, nucleocapsid, International, limit of detection, COVID-19 virus, RdRP, RNA-dependent RNA polymerase, public health emergency, respiratory, quantification, E-gene, standard curve, Dilution, acute respiratory syndrome, supplementary material, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, E gene, viral loads, Roche Diagnostics, RT-PCR kits, RT-PCR kit, division, LLOQ, limit, Result, evaluated, provided, calculated, 【제목키워드】 RT-PCR detection,