A simple, safe and sensitive method for SARS‐CoV‐2 inactivation and RNA extraction for RT‐qPCRRT-qPCR을 위한 SARS-CoV-2 비활성화 및 RNA 추출을 위한 간단하고 안전하며 민감한 방법Original Article Published on 2021-03-172022-09-01 Journal: Apmis [Category] Fulltext, SARS, 진단, 치료기술, [키워드] Acidic Analysis B2M can be used chloroform circumvent clinical microbiology detect determine diagnostic test diagnostic tests Dilution dilution sery downstream equipment expand guanidinium inactivated inactivation Laboratory molecular microbiology mRNA nasopharyngeal Oropharyngeal pandemic phenol protocol rapid diagnostic methods reagent RNA extraction RNA extraction kit RNA purification kits RT‐qPCR Safe SARS‐CoV‐2 SARS‐CoV‐2 SARS‐CoV‐2 RT‐qPCR second sensitivity Swab swab samples tested threshold Viral Viral RNA Virology virus virus inactivation [DOI] 10.1111/apm.13123 PMC 바로가기 [Article Type] Original Article
Neutralization of SARS-CoV-2 with IgG from COVID-19-convalescent plasmaCOVID-19 회복기 혈장에서 IgG로 SARS-CoV-2 중화Article Published on 2021-03-102022-09-01 Journal: Scientific Reports [Category] SARS, 임상, 진단, 치료기술, 치료제, [키워드] administer antibody approach change clinical utility COVID-19 patient COVID-19 patients detectable Dilution elicit endpoints examined fraction greater IgG immune response Immunotherapy inhibited neutralization activity Neutralizing activity plasma plasma sample plasmas producing quantification reduced required SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected patients surrogate the patient viral infection while [DOI] 10.1038/s41598-021-84733-5 PMC 바로가기 [Article Type] Article
Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2SARS-CoV-2의 민감하고 특이적이고 신속한 검출을 위한 동결건조 CRISPR-Cas12 분석 평가Article Published on 2021-03-052022-09-01 Journal: Viruses [Category] SARS, 진단, 치료기술, [키워드] 95% confidence interval collected Concordance COVID-19 CRISPR cross-reaction cycle threshold demonstrated detection Diagnosis diagnostic tests Dilution dilutions equipment evaluated facilitate fluorescence detection heat heat-inactivated virus initial isothermal amplification lysis buffer Molecular diagnosis nasopharyngeal sample Nasopharyngeal samples Nasopharyngeal swab nasopharyngeal swabs negative sample Pathogens performed positive sample qPCR Rapid reaction reduce respiratory respiratory pathogen reverse transcription RNA sample RNA samples RT-qPCR SARS-CoV-2 SARS-CoV-2 detection sensitive Specific tested [DOI] 10.3390/v13030420 PMC 바로가기 [Article Type] Article
Development and validation of an LC-MS/MS method for determination of hydroxychloroquine, its two metabolites, and azithromycin in EDTA-treated human plasmaResearch Article Published on 2021-03-052022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] active against acute respiratory syndrome antimalarial drug blood sample clinical study conditions coronavirus COVID-19 pandemic develop development Dilution emergency response ESI freeze-thaw cycles HCQ Human plasma Hydroxychloroquine injected Ion LC-MS/MS system less Liquid chromatography m/z matrix effect metabolite metabolites normalized overcome pandemic quantification quantitation ranged reported Result Sample SARS-CoV-2 selected Tandem mass spectrometry temperature Total Volume was used were used [DOI] 10.1371/journal.pone.0247356 PMC 바로가기 [Article Type] Research Article
Group testing as a strategy for COVID-19 epidemiological monitoring and community surveillanceCOVID-19 역학 모니터링 및 지역사회 감시를 위한 전략으로 그룹 테스트Research Article Published on 2021-03-042022-09-12 Journal: PLoS Computational Biology [Category] SARS, 진단, [키워드] Analysis Asymptomatic individuals Community COVID-19 datasets diagnostic Dilution Early detection Efficiency Epidemic epidemiological estimate False negative false negatives General population group individual infected individual infected individuals measure nursing homes one individual outbreak pool pooling Positive test Prevalence proportion reduced reduction RT-qPCR Sample SARS-CoV2 Screening sensitivity statistical model Surveillance tested the mean unique Viral load virus [DOI] 10.1371/journal.pcbi.1008726 PMC 바로가기 [Article Type] Research Article
Differential Effects of Antiseptic Mouth Rinses on SARS-CoV-2 Infectivity In VitroArticle Published on 2021-03-012022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] alcohol antiseptic antiseptics Antiviral antiviral activity Asymptomatic individuals benefit blocked Cell cells cellular damage centrifugation coronavirus cytotoxic effect cytotoxicity detectable Dilution Effect exhibited greater hydrogen peroxide Impact inactivated Infection Infectivity Listerine moderate mouth mouth rinses outcome pseudotyped SARS-CoV-2 reduce reducing reduction replication-competent SARS-CoV-2 Saliva SARS-CoV-2 SARS-COV-2 infection significantly Spread suppress treated viability viral infectivity Viral load virus virus infectivity virus spread viruses [DOI] 10.3390/pathogens10030272 PMC 바로가기 [Article Type] Article
Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients입원하지 않은 COVID-19 환자의 SARS-CoV-2 및 계절성 코로나바이러스에 대한 항체 반응Research Article Published on 2021-02-242022-09-10 Journal: mSphere [Category] MERS, 진단, 치료기술, 치료법, 치료제, [키워드] 229E addition addressed ADE antibody Antibody Response antibody response against Antibody responses Antibody-dependent enhancement antibody-dependent enhancement (ADE) Asymptomatic breadth conducted contribute coronavirus coronavirus 229E correlated Course COVID-19 cross-protection detect develop diagnostic diagnostic test difficulty Dilution disease experiments highlight Hospitalization human cell human cells Immunity individual infected individuals Infection involved majority marker Mild neutralization Neutralizing activity Neutralizing antibodies Neutralizing antibody response neutralizing antibody responses nucleocapsid Patient phenomenon positive predictor quantified question receptor binding site response S protein SARS-CoV-2 SARS-CoV-2 neutralization seasonal seasonal coronaviruses sera serological Serological assay serological survey Serum Neutralization Spike protein surrogate markers symptomatic targets the S protein the spike protein to define Vaccine development Virus neutralization western blot [DOI] 10.1128/mSphere.01145-20 PMC 바로가기 [Article Type] Research Article
Rapid Assessment of SARS-CoV-2 Transmission Risk for Fecally Contaminated River WaterArticle Published on 2021-02-222022-10-28 Journal: ACS Es&t Water [Category] COVID-19, [키워드] acute respiratory syndrome approach approaches assessment available data Contaminated coronavirus country Critical decrease Dilution droplet Epidemiology example Fecal–oral transmission fecal−oral identifying in vitro data infection rate infectious dose outbreak Outbreaks Rapid recommendation Research risk risk of transmission river SARS-CoV-2 sewage survival temperature Transmission transmission route used to evaluate Viral load water water transmission [DOI] 10.1021/acsestwater.0c00246 PMC 바로가기 [Article Type] Article
Experimental characterization of speech aerosol dispersion dynamics음성 에어로졸 분산 역학의 실험적 특성화Article Published on 2021-02-172022-08-31 Journal: Scientific Reports [Category] MERS, 치료제, [키워드] activity acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 aerosol Aerosols coronavirus coughing coughs Dilution disease exhibited followed by Immediate inhalation not correlated Particle Particles pathway penetrated Penetrating penetration penetrations prevented production rate production rates quantified relative respiratory Respiratory tract diseases Result risk Risk factors SARS-CoV-2 severe acute respiratory syndrome Coronavirus sneezing Structure subject Syllable tested Transmission vocalization [DOI] 10.1038/s41598-021-83298-7 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Subgenomic N ( sgN ) Transcripts in Oro-Nasopharyngeal Swabs Correlate with the Highest Viral Load, as Evaluated by Five Different Molecular MethodsArticle Published on 2021-02-122022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] amplification applied assays Biomarker CE-IVD COVID-19 pandemic detect diagnostic diagnostics digital PCR Dilution Effects evaluated FIVE genomic IMPROVE information Laboratory load marker medium molecular test PBS question ranged RdRP replicate representing respond Result RNA RNA transcript RT-qPCR SARS-CoV-2 SARS-CoV-2 subgenomic regions SARS-CoV-2 viral Severe infection Spread subgenomic transcript swabs target target gene TMB transcript Viral load [DOI] 10.3390/diagnostics11020288 PMC 바로가기 [Article Type] Article