Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveysArticle Published on 2022-12-012022-11-15 Journal: Diagnostic microbiology and infectious disease [Category] COVID19(2023년), SARS, 진단, [키워드] anti-spike protein IgG antibody approach Brazil change changes in Community-based cohort conducted decrease Dilution dilutions ELISA evaluate fitting IgG ELISA Immunity incidence increase interpolated MONITOR normalized optical optical density pandemic Participants predicted reduce reliability resource SARS-CoV-2 sera serial dilutions serology Serosurvey significantly titers Titers. was performed [DOI] 10.1016/j.diagmicrobio.2022.115807 PMC 바로가기
Practical considerations to establish a validated platform for pooled detection of SARS-CoV-2 by droplet digital PCRArticle Published on 2022-11-042022-11-15 Journal: PLoS ONE [Category] SARS, 치료기술, [키워드] addition affected amplification analyzed approach Blood carried COVID-19 pandemic Critical Ct value detect detection diagnostic diagnostic capabilities digital PCR Dilution droplet False negative Health hospital Infectious disease inhibitors invasive Laboratory less limitation Nasopharyngeal samples parameter PCR platform pool positive sample reagent reagents reduce reducing resources resulting risk Saliva SARS-CoV-2 sensitivity the SARS-CoV-2 virus load was performed [DOI] 10.1371/journal.pone.0271860 PMC 바로가기
New insights into antibody levels against SARS-CoV-2 for healthcare personnel vaccinated with tozinameran (Comirnaty)Article Published on 2022-11-032022-11-15 Journal: PLoS ONE [Category] SARS, 진단, [키워드] activated addition addressed administration Alto Andújar antibody booster dose COVID-19 determine Dilution Elecsy Evolution exhibited Germany Guadalquivir healthcare personnel Healthcare professional humoral IgG in vitro infected patients material Neutralizing antibodies New non-infected pandemic platform Roche SARS-CoV-2 SARS-CoV-2 vaccine second dose serum sample Spike protein subject Thermo Fisher Scientific titer total antibody vaccination Vaccine Volunteer volunteers [DOI] 10.1371/journal.pone.0276968 PMC 바로가기
Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspectiveArticle Published on 2022-10-202022-11-15 Journal: PLoS ONE [Category] 치료제, [키워드] ABI ABI 7500 Applied Biosystem approach Bio-rad comparable comparison Conventional COVID-19 COVID-19 sample detection did not produce Dilution human RP mRNA multiplex N1 and N2 optimization PCR PCR amplification Perspective positive control Precision protocol real time RNase P RT-PCR SARS CoV-2 sensitivity Sensitivity and specificity sequence specificity Standard System the median time touch was done was performed [DOI] 10.1371/journal.pone.0276464 PMC 바로가기
Heterologous booster COVID-19 vaccination elicited potent immune responses in HCWsArticle Published on 2022-10-012022-11-15 Journal: Diagnostic microbiology and infectious disease [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] administration antibody Antibody titer BNT162b2 booster booster dose CoronaVac COVID-19 COVID-19 infection COVID-19 vaccination Dilution dose doses effective elicited evaluate HCW HCWs Health care worker IgG antibody titer IgG level immune response inactive individual mRNA vaccine plaque reduction neutralization Risk factors SARS-CoV-2 vaccine the antibody response the median vaccination Vaccine were measured [DOI] 10.1016/j.diagmicrobio.2022.115758 PMC 바로가기
Analytical sensitivity of the Rapid Antigen Test kits for detection of SARS-CoV-2 Omicron variant BA.2 sublineageSARS-CoV-2 Omicron 변이체 BA.2 하위 계통의 검출을 위한 Rapid Antigen Test 키트의 분석 감도Article Published on 2022-10-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus analytical sensitivity B.1.1.529 BA.2 Chain Reaction collected comparable coronavirus COVID-19 COVID-19 patient detect determined by Dilution evaluated lack limit of detection omicron polymerase chain reaction Rapid rapid antigen test reference method reverse transcription Reverse transcription-polymerase chain reaction SARS-CoV-2 selected sensitivity specimen Spread Test variant was determined was used [DOI] 10.1002/jmv.27908 PMC 바로가기 [Article Type] Article
Activity of ertapenem/zidebactam (WCK 6777) against problem EnterobacteralesArticle Published on 2022-09-302022-11-16 Journal: Journal of Antimicrobial Chemotherapy [Category] SARS, 비임상, [키워드] 1:1 active against activity administration breakpoint Dilution E. coli Escherichia coli evaluated experiments group healthcare increasingly indicated Infection inhibited isolate Klebsiella KPC Laboratory material MDR National nosocomial Outpatient over Parenteral pathogen Patient proportion regimen SARS-CoV-2 secondary submitted Support susceptibility Trial will drive [DOI] 10.1093/jac/dkac280 PMC 바로가기
A novel assessment method for COVID-19 humoral immunity duration using serial measurements in naturally infected and vaccinated subjectsArticle Published on 2022-09-292022-11-15 Journal: PLoS ONE [Category] SARS, 변종, 진단, [키워드] antibodies antibody Antigen applied Characteristics Collecting COVID-19 defined determine Dilution dilutions dose doses help humoral Humoral immunity immune response Infection information intensity linear long-lasting MONITOR Pfizer Prevent profile protective immunity quantification quantitative assay RBD reactivity Reinfection Sample SARS-CoV-2 SARS-CoV-2 antigens serological serology test serum sample subject two group vaccination Vaccination strategies Vaccine variant Viral antigen was used were used [DOI] 10.1371/journal.pone.0274553 PMC 바로가기
Biomarkers selection for population normalization in SARS-CoV-2 wastewater-based epidemiologyArticle Published on 2022-09-012022-11-15 Journal: Water research [Category] SARS, 바이오마커, [키워드] 5-hydroxyindoleacetic acid acute respiratory syndrome addition approach Biomarker Biomarkers CDC changes in city Community complement Concentration contributing to coronavirus coronavirus disease correlation COVID-19 creatinine Critical demonstrated Dilution distribution epidemiological Epidemiology evaluated FIVE help high accuracy investigated Local metabolite Mild normalized outbreak Paraxanthine plant Population Population biomarker Population normalization Prevalence reported SARS-CoV-2 SARS-CoV2 selected significantly tested the SARS-CoV-2 Treatment utility Variability Viral load viral loads virus Volume Wastewater-based epidemiology [DOI] 10.1016/j.watres.2022.118985 PMC 바로가기
Accurate quantification of SARS-CoV-2 RNA by isotope dilution mass spectrometry and providing a correction of reverse transcription efficiency in droplet digital PCRArticle Published on 2022-09-012022-11-16 Journal: Analytical and bioanalytical chemistry [Category] SARS, 유전자 메커니즘, [키워드] Accuracy acute respiratory syndrome analyzed approach caused confirmed cases coronavirus COVID-19 deaths demonstrated digested digestion Digital digital PCR Dilution Efficiency IDMS measure monophosphate novel coronavirus disease nucleotide Quality control quantification Quantitative reference material reliability reverse transcription RNA RNA molecule RT reaction SARS-CoV-2 SARS-CoV-2 RNA [DOI] 10.1007/s00216-022-04238-6 PMC 바로가기