Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: A reliable, faster and economical methodSARS CoV-2 감염에 대한 풀링된 RNA 샘플 역전사효소 실시간 PCR 분석: 신뢰할 수 있고 빠르고 경제적인 방법Research Article Published on 2020-07-302022-08-13 Journal: PLoS ONE [Category] MERS, SARS, 유전자 메커니즘, [키워드] chain China Cluster Corona virus country COVID-19 Ct value demonstrated detect diagnosis of COVID-19 diagnosis of SARS-CoV-2 diagnostic capabilities disease E gene evaluate expand Extracted faster help hour Infection not affected novel objective pandemic PCR PCR assay Pneumonia pneumonia case pneumonia cases pool pooling positive sample protocol Randomly reagent reagents real time reduce Result RNA RNA sample RNA samples RT-qPCR SARS CoV SARS CoV-2 SARS-COV-2 infection Study design transcriptase Transmission virus Virus Disease Wuhan [DOI] 10.1371/journal.pone.0236859 PMC 바로가기 [Article Type] Research Article
Health Surveillance and Response to SARS-CoV-2 Mass Testing in Health Workers of a Large Italian Hospital in Verona, VenetoArticle Published on 2020-07-152022-10-30 Journal: International Journal of Environmental Research an [Category] COVID-19, SARS, [키워드] 95% CI administration age China close contact Community confirmed case COVID COVID-19 COVID-19 outbreak cumulative diffusion Evidence Gender Health Health Service health worker Healthcare workers hospital incidence Infection Isolation Italian Italian region Italy knowledge maintain mass Mass screening nasopharyngeal swabs nosocomial not affected Oropharyngeal participant PCR predictor Prevent provided response returned risk SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections specimen surgical Surveillance surveillance data Swab test tested Testing Veneto Verona viral gene worker [DOI] 10.3390/ijerph17145104 PMC 바로가기 [Article Type] Article
Evaluation of psychological stress in scientific researchers during the 2019–2020 COVID-19 outbreak in ChinaGlobal Health Published on 2020-07-062022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] affected benefit candidate caused change China Chinese Complete conducted coronavirus disease COVID-19 COVID-19 epidemic COVID-19 outbreak COVID-19 research experiment Government Grounding indicated introduced measure not affected outbreak participant Participants Pneumonia populations Prevent Psychological psychological pressure Psychological stress question questionnaire receiving Research researcher respondent Result spread of COVID-19 Stress stress level Support survey was obtained [DOI] 10.7717/peerj.9497 PMC 바로가기 [Article Type] Global Health
Impact of COVID-19 outbreak on ischemic stroke admissions and in-hospital mortality in North-West SpainResearch Published on 2020-06-262022-10-29 Journal: International Journal of Stroke [Category] COVID-19, MERS, SARS, [키워드] Admission Admitted Patient analyzed change country COVID-19 COVID-19 epidemic COVID-19 outbreak COVID-19 pandemic decrease Epidemic explained hospital Impact in-hospital mortality increase in Ischemic Stroke Mortality multicenter no difference not affected pandemic Patient proportion recorded Region regions Result retrospective severity significantly Spain Spanish stroke stroke care study period tertiary referral hospital [DOI] 10.1177/1747493020938301 PMC 바로가기 [Article Type] Research
Do COVID-19 and SARS Gene Complexities and Variations Help Overcome the Knowledge Gap?Infectious Disease Published on 2020-06-042022-10-31 Journal: Cureus [Category] COVID-19, [키워드] acute respiratory syndrome caused complexity Control coronavirus Coronaviruses COVID-19 develop disease Diseases effective Epidemics explain HCoV-229E HCoV-OC43 help Human human coronavirus human coronavirus 229E human coronavirus OC43 human gene illness illnesses immune response immune system individual Infection intestinal not affected pathogen pathogenic pre-existing immunity reflected replicate Research respiratory tract respond risk RNA virus SARS SARS-CoV-2 similarities Spread Symptom Symptoms the disease Transmission Vaccine Variation virus [DOI] 10.7759/cureus.8439 PMC 바로가기 [Article Type] Infectious Disease
Provision of obstetrics and gynaecology services during the COVID‐19 pandemic: a survey of junior doctors in the UK National Health ServiceOriginal Article Published on 2020-05-272022-10-28 Journal: BJOG : an international journal of obstetrics and [Category] COVID-19, MERS, SARS, [키워드] Abstract acute phase Analysis Antenatal Audit Care clinics coronavirus coronavirus disease Coronavirus disease 2019 COVID‐19 disrupting doffing donning evaluate gynaecology Health Service healthcare majority MOST National National Health Service not affected objective Obstetric obstetrics offered oncology pandemic participant pathway Population PPE protocol Quantitative analysis question reduced reduction in reported response Result Safe setting survey women women's health care [DOI] 10.1111/1471-0528.16313 PMC 바로가기 [Article Type] Original Article
Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent mannerArticle Published on 2020-02-242022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, [키워드] antiviral treatments Cell cell entry cell-to-cell fusion contribute Culture demonstrated develop Efficiency Entry epidemic diarrhea infected cell infected cells investigated knowledge mechanism medium membrane fusion MOST not affected PDCoV PEDV Porcine deltacoronavirus promote pseudovirus entry assays Replication Transmission trypsin uninfected cell Vaccine viral entry viral replication virus virus production virus release [DOI] 10.1080/22221751.2020.1730245 PMC 바로가기 [Article Type] Article
Establishment of Primary Transgenic Human Airway Epithelial Cell Cultures to Study Respiratory Virus–Host InteractionsArticle Published on 2019-08-132022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] airway epithelium Cell Cell culture Combined Culture cultures expression facilitate Factor fluorescence-activated cell sorting fluorescent functional Genetic hAEC HCoV-229E Host host gene expression Human human airway epithelial human airway epithelial cell cultures human coronavirus 229E human respiratory epithelium human respiratory virus in vitro inhibitor innate immune response Interaction knockdown lentiviral Modification modulated molecular not affected platform primary cell primary human cell Protein protocol reporter respiratory respiratory syncytial virus respiratory virus respiratory viruses target cells transgenic unique virus infection Virus-host interactions virus–host interaction Y-27632 [DOI] 10.3390/v11080747 PMC 바로가기 [Article Type] Article
Targeting Membrane-Bound Viral RNA Synthesis Reveals Potent Inhibition of Diverse Coronaviruses Including the Middle East Respiratory Syndrome VirusResearch Article Published on 2014-05-292022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] accompanied accumulating antiviral activity antiviral drug antiviral intervention cellular membrane complex component Compound concerning conserved Coronaviral coronavirus coronavirus 229E Coronavirus infection Coronavirus replication Coronaviruses Culture early phase epithelia genome replication hallmark highly pathogenic Host host cell cytoplasm implicated Including infections inhibit inhibition inhibitor knowledge membrane membrane vesicle membrane-bound membranes MERS-CoV MERS–CoV Middle East mutant Mutation non-structural protein not affected positive potent raise recruitment reduced replicate Replication representing respiratory respiratory syndrome coronavirus RNA RNA synthesis RNA virus RNA viruses SARS-CoV screened Substitution synthesis target targeting Transcription Treatment viral life cycle viral replication viral RNA synthesis virus virus entry zoonotic [DOI] 10.1371/journal.ppat.1004166 PMC 바로가기 [Article Type] Research Article
Molecular Mechanisms of Hypoxic Responses via Unique Roles of Ras1, Cdc24 and Ptp3 in a Human Fungal Pathogen Cryptococcus neoformans인간 곰팡이 병원체 Cryptococcus neoformans에서 Ras1, Cdc24 및 Ptp3의 고유한 역할을 통한 저산소 반응의 분자 메커니즘Research Article Published on 2014-04-242022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Actin affected Analysis biological processe biological processes C. neoforman C. neoformans CAMP caused Cdc42 central gene chromosome Compound Concentration condition conserved Critical Cryptococcus Cryptococcus neoforman Cryptococcus neoformans cytoskeletal Deletion downstream eight ergosterol eukaryotic cells expression of gene family function fungal genes encoding genetic analysis genotoxic growth growth defect growth retardation GTPase guanine Human human host hypoxia hypoxic hypoxic condition hypoxic conditions indicated inhibit inhibitor involved maintenance molecular mutant not affected nucleotide osmotic oxygen pathogen pathway Pathways phosphatase phosphatases Phosphorylation Protein protein translation Rac1 Rac2 regulate required rescued response Rho GTPase RNA RNA sequencing role sequence similarity single cells strain Strains suggested suppressor These cells Tolerance unique wild-type strain [DOI] 10.1371/journal.pgen.1004292 PMC 바로가기 [Article Type] Research Article