Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice조작된 SARS-CoV-2 수용체 결합 도메인은 효모의 제조 가능성과 마우스의 면역원성을 향상시킵니다.403 Published on 2021-09-212022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 변종, 진단, [키워드] ACE2 Antigen approved binding binding domain Candidates Cell country COVID-19 COVID-19 pandemic domain dose doses exhibit exhibited hamster hamsters high-affinity binding immune response immunogenicity IMPROVE Intervention limit manufacturability mice microbial MOST other diseases Pichia pastoris presentation Protein protein vaccine raise raised RBD Receptor-binding domain Recombinant protein recombinant proteins reduced reported Safe SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 variant sequence sequences Significance single dose Subunit vaccine subunit vaccines the RBD the receptor-binding domain the SARS-CoV-2 Vaccine vaccine candidate Vaccines variant Viral viral challenge viral entry virus VLP Volume weight loss Wuhan-Hu-1 yeast [DOI] 10.1073/pnas.2106845118 PMC 바로가기 [Article Type] 403
Kinetics of Nucleocapsid, Spike and Neutralizing Antibodies, and Viral Load in Patients with Severe COVID-19 Treated with Convalescent Plasma심각한 Covid-19 환자의 핵형 캡시드, 스파이크 및 중화 항체 및 바이러스 부하의 동역학, 수밀 혈장으로 처리 된 환자의 동역학Clinical Trial Published on 2021-09-152022-09-01 Journal: Viruses [Category] SARS, 임상, 진단, 치료법, [키워드] Analysis antibody antibody kinetics Antibody Response Antibody titers baseline binding binding and neutralizing antibodies binding domain changes in clearance cohort of patient Complete convalescent plasma COVID-19 CP infusion demonstrated develop Dexamethasone effective enrolled evaluate Humoral response immunological Infection Kinetics kinetics of antibody load median meta-analyses morbidity and mortality Mortality Neutralizing antibodies neutralizing antibody Neutralizing antibody titer neutralizing antibody titers nucleocapsid nucleocapsid protein pandemic Patient peak levels plasma Protein pseudotype rapid increase Receptor-binding domain recipient recipients reduction Research RNA robust Safe SARS-CoV-2 SARS-CoV-2 PCR SARS-CoV-2 protein SARS-CoV-2 proteins shown spike Spike protein symptom onset the receptor-binding domain titers trajectory Treatment trimeric Trimeric spike protein Viral viral loads Virological virus virus clearance [DOI] 10.3390/v13091844 PMC 바로가기 [Article Type] Clinical Trial
Infectious SARS-CoV-2 in Exhaled Aerosols and Efficacy of Masks During Early Mild Infection호기 에어로졸의 전염성 SARS-CoV-2와 경증 초기 감염 시 마스크의 효능Major Article Published on 2021-09-142022-09-10 Journal: Clinical Infectious Diseases: An Official Publicat [Category] SARS, 변종, 치료기술, [키워드] 95% CI 95% confidence interval aerosol Aerosol generation Aerosols Airborne infection Alpha Alpha variant Anti-spike antibodies binding domain Blood breath collected confounders Control COVID-19 case COVID-19 cases Culture cultured virus enrolled exhaled breath aerosol Face masks Impact increase in Mask Masks not significantly different participant quantified recruited reduced remained Respirator respirators Result Saliva SARS-CoV-2 SARS-CoV-2 epidemiology SARS-CoV-2 RNA SARS-CoV-2 variants sequenced sera seronegative surgical swabs vaccination rate vaccination rates variant variants Viral Viral RNA viruses [DOI] 10.1093/cid/ciab797 PMC 바로가기 [Article Type] Major Article
Structural and energetic profiling of SARS-CoV-2 receptor binding domain antibody recognition and the impact of circulating variantsSARS-COV-2 수용체 결합 도메인 항체 인식의 구조적 및 에너지 프로파일 링 및 순환 변이체의 영향Research Article Published on 2021-09-072022-09-10 Journal: PLoS Computational Biology [Category] SARS, 변종, 진단, [키워드] affected Alpha Analysis antibody atomic Author B.1.1.7 B.1.617.2 binding binding domain circulating variant Clustering Community complex computational modeling Contact COVID-19 pandemic dataset Delta described determinant disrupt epitope epitope conservation evade feature group high resolution highlight identify immune response immune responses less molecular monoclonal antibodies monoclonal antibody Mutation mutations natural infection Neutralizing antibodies performed predicted Protective antibody protective antibody responses RBD Receptor binding domain Research residue residues SARS-CoV-2 pandemic SARS-CoV-2 receptor SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus spike glycoprotein Spread Structural analysis Structure structures target the SARS-CoV-2 the SARS-CoV-2 virus therapeutic Unsupervised clustering Vaccine Vaccine design Vaccines variants Viral viral variant Viral variants was used [DOI] 10.1371/journal.pcbi.1009380 PMC 바로가기 [Article Type] Research Article
Evaluation of the SARS-CoV-2 Antibody Response to the BNT162b2 Vaccine in Patients Undergoing Hemodialysis혈액투석을 받는 환자에서 BNT162b2 백신에 대한 SARS-CoV-2 항체 반응 평가Research Published on 2021-09-022022-09-10 Journal: JAMA Network Open [Category] SARS, 진단, 치료법, [키워드] anti-RBD Anti-spike Antibody Response baseline binding domain BNT162b2 BNT162b2 vaccine Canada Care center Chain Reaction Chronic hemodialysis clinical trial cohort study conducted Control controls convalescent serum COVID-19 COVID-19 vaccination COVID-19 vaccine COVID-19 vaccines defined dose evaluate exceeded exposure finding Health Health care Health care worker health care workers Health policy Hemodialysis high mortality rate humoral Humoral response IgG antibodies immune response Importance individuals interquartile range kidney Kidney disease levels of antibody Maintenance hemodialysis measure median median age nucleocapsid protein objective occurred of BNT162b2 participant Patient patient population Pfizer Pfizer-BioNTech Point polymerase chain polymerase chain reaction public health randomized clinical trials reached receipt receiving Receptor binding domain Relevance response Result reverse transcriptase reverse transcriptase polymerase chain reaction testing robust SARS-CoV-2 IgG SARS-CoV-2 IgG antibodies SARS-CoV-2 IgG antibody SARS-COV-2 infection second dose Seroconversion Serologic response setting single dose single-center Spike protein Survivors the median the SARS-CoV-2 the spike protein Toronto transcriptase vaccination vaccination schedule Vaccinations Vaccine vaccine dose vaccine doses vaccine immunogenicity were measured with COVID-19 [DOI] 10.1001/jamanetworkopen.2021.23622 PMC 바로가기 [Article Type] Research
Longitudinal observation of antibody responses for 14 months after SARS-CoV-2 infectionSARS-CoV-2 감염 후 14개월 동안 항체 반응에 대한 종단 관찰Observational Study Published on 2021-09-012022-09-11 Journal: Clinical immunology (Orlando, Fla.) [Category] SARS, 임상, 진단, 치료법, [키워드] Analysis analyzed anti-S Anti-spike Antibody assays Antibody Response Antibody responses Antibody titer Antibody titers Better binding domain carried Clinical course clinical history coronavirus COVID19 demographics different time points false-negative rate highlight Humoral immunity IgG IgG antibody IgG antibody titer IgG antibody titers immunoassay immunoassays implementation Italy Longevity longitudinal longitudinal analysis Loss of Smell loss of smell and taste magnitude natural infection Neutralizing antibodies observation positive Protein RBD IgG Re-infection Re-infections recovered COVID-19 patient recovered patients S-RBD SARS-CoV-2 SARS-COV-2 infection serological test Serological tests seronegative significantly higher subject Symptom Taste tested the disease vaccination were used [DOI] 10.1016/j.clim.2021.108814 PMC 바로가기 [Article Type] Observational Study
The impact of spike N501Y mutation on neutralizing activity and RBD binding of SARS-CoV-2 convalescent serumSARS-CoV-2 회복기 혈청의 중화 활성 및 RBD 결합에 대한 스파이크 N501Y 돌연변이의 영향Article Published on 2021-09-012022-09-11 Journal: EBioMedicine [Category] MERS, SARS, 변종, 진단, [키워드] 95% CI Amino acid amino acids ANOVA Anti-RBD IgG Anti-S1 antibody Antibody binding assays B.1.1.7 B.1.351 binding binding domain clinician collected convalescent sera convalescent serum COVID-19 COVID-19 patient determine enzyme immunoassay evaluated geometric geometric mean geometric mean titer GMT Hong Kong IgG isolates K417N Lineage mean difference Michael microneutralization N501Y N501Y mutation neutralization Neutralizing activity neutralizing antibody Neutralizing antibody Spike protein receptor binding domain NSP2 one-way ANOVA Patient positive Protein RBD RBD binding Receptor binding Receptor binding domain recovered COVID-19 patient reduced Richard SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 lineage SARS-CoV-2 N501Y variant B.1.1.7 sensitivity sera serum service significantly spike Spike protein Spike receptor binding domain Spread statistically susceptibility symptom onset tested variant variants virus virus variant virus variants viruses VoC VOC. was determined wild-type [DOI] 10.1016/j.ebiom.2021.103544 PMC 바로가기 [Article Type] Article
Mechanical activation of spike fosters SARS-CoV-2 viral infection스파이크의 기계적 활성화는 SARS-CoV-2 바이러스 감염을 촉진합니다Article Published on 2021-08-312022-09-11 Journal: Cell Research [Category] MERS, SARS, 변종, 진단, [키워드] accelerate ACE2 ACE2 binding Activation Anti-S1 antibody binding binding domain blocking antibody caused complex convalescent COVID-19 patient convalescent COVID-19 patients COVID-19 pandemic D614G Detachment domain faster force host cell host cell membrane Host receptors Invasion Mechanical Molecular biology mutant Neutralizing outbreak Prevent Receptor-binding domain recognize reduce Rotation S1 subunit S1/S2 S2 subunit SARS-CoV-2 SARS-CoV-2 viral infection SARS2 Spike protein structural biology tensile tensile force therapeutic strategy variant Viral viral entry [DOI] 10.1038/s41422-021-00558-x PMC 바로가기 [Article Type] Article
Development of Effective Therapeutic Molecule from Natural Sources against Coronavirus ProteaseArticle Published on 2021-08-302022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, MERS, SARS, [키워드] addition Administered amino acid residue Antiviral agents binding binding domain can be used complex complexes coronavirus disease correlated Course COVID-19 COVID-19 patients demonstrated development disease docked docking docking result docking score drug candidate drug design effective exhibited flavonols Free energy calculations in silico in silico Approach inhibitor inhibitory Interaction investigated involved less Ligand M pro MPro MDs MM/GBSA molecular Molecular dynamics simulation molecular target molecule Mutation myricetin Myricitrin natural natural products protein-ligand interaction reduced Remdesivir residue RMSD RMSF rutin SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 Mpro selected Source stability Treatment Vaccine [DOI] 10.3390/ijms22179431 PMC 바로가기 [Article Type] Article
Simultaneous evaluation of antibodies that inhibit SARS-CoV-2 variants via multiplex assayResource and Technical Advance Published on 2021-08-232022-10-30 Journal: JCI Insight [Category] COVID-19, SARS, [키워드] ACE2 receptor ACE2-RBD Angiotensin-converting enzyme antibody Antibody therapeutics binding domain candidate complementary COVID-19 domains Effectiveness facilitate functional Immune escape immune escape mutations immunoglobulins inhibit SARS-CoV-2 inhibitory monoclonal antibody Multiplex assay N439K N501Y natural infection neutralizing antibody neutralizing capacity RBD RBD variant S477N SARS-CoV-2 receptor SARS-CoV-2 research serological Simultaneous Surveillance the SARS-CoV-2 vaccination Vaccine variant [DOI] 10.1172/jci.insight.150012 PMC 바로가기 [Article Type] Resource and Technical Advance