Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis막내 단백질 분해의 조절이 Bacillus subtilis에서 포자 형성 동안 기질 게이팅에 의해 매개된다는 증거Research Article Published on 2018-11-072022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activate Activation active form association Bacillus subtilis catalytic center catalyzed CBS Cell cells cleavage cleave cleaving co-immunoprecipitation conformation conformational change conserved Critical cystathionine cytoplasmic daughter cells domain enzyme Evidence Extracellular family member fluorescence microscopy forespore Gene Expression homolog immunoprecipitation in vivo inactive information Interaction interaction site interior late stage mechanism membrane membrane protein Methanocaldococcus Morphological mother mother cell open pathway Pathways protease proteolysis proteolytic activation Regulation remained secreted Signal transduction Signaling signaling pathway Space sporulation Support transcription factor transducing Trigger triggers [DOI] 10.1371/journal.pgen.1007753 PMC 바로가기 [Article Type] Research Article
Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysisArticle Published on 2018-10-242022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] ACE2 acute respiratory syndrome Analysis angiotensin-converting enzyme 2 bind binding cleavage cleaved conformational conformational change coronavirus cryo-EM glycoprotein Host host cell host cells host membranes host protease host protein Host receptors human betacoronavirus Interaction Mutation pathogenic pre-fusion Prevent receptor S1/S2 cleavage site SARS-CoV SARS-CoV S secondary cleavage spike trimeric trypsin undergo viral entry viral spike [DOI] 10.1038/s41598-018-34171-7 PMC 바로가기 [Article Type] Article
Transmembrane Domains of Highly Pathogenic Viral Fusion Proteins Exhibit Trimeric Association In VitroObservation Published on 2018-04-182022-10-30 Journal: mSphere [Category] Coronavirus, MERS, [키워드] acute respiratory syndrome approach cell membrane conformational change contribute coronavirus demonstrated domain Ebola virus ectodomain element event Factor fusion fusion protein glycoprotein human pathogen implicated indicate Influenza Influenza virus Interaction membrane-bound molecular Mutation observation occur prefusion promote Protein protein stability Proteins rabies Rabies virus residue SARS SARS CoV sedimentation equilibrium stability therapeutic thought TMD TMDs transmembrane domain trimeric ultracentrifugation undergo understanding viral entry viral envelope viral family virus [DOI] 10.1128/mSphere.00047-18 PMC 바로가기 [Article Type] Observation
Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent MannerResearch Article Published on 2014-11-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] Cell cell entry Clathrin-mediated endocytosis cleavage conformational change coronavirus CoV CoVs demonstrated endocytosed endosomal maturation endosomal pH entry pathway expressing furin furin cleavage site fusion fusion peptide fusion protein gene silencing Genome Host host cell host cell membrane induce Infection influenza A virus inhibited introduced investigated late endosomes less lysosomal protease lysosomal proteases lysosome membrane fusion MERS MERS-CoV MHV microenvironment mouse hepatitis murine hepatitis virus negatively affected occur pathogen plasma membrane predicted previous study promote protease Protease inhibitor Protein proteolytic cleavage Proteolytic processing reporter required S protein shown siRNA Specific the S protein upstream vesicular stomatitis virus virus while zoonotic [DOI] 10.1371/journal.ppat.1004502 PMC 바로가기 [Article Type] Research Article
The SARS Coronavirus 3a protein binds calcium in its cytoplasmic domainArticle Published on 2014-08-102022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] aligned amino acid residue amino acids Analysis bind binding calcium cause conformational change Cyto3a protein cytoplasmic domain E. coli ENCODE enzyme expressed form Genome Glutamine His-tagged Hypothesis in viral in vitro in vivo involved ion channel Leptospira Open reading frame ORF, open reading frame Pathogenesis Plasmodium falciparum positive predicted Protein Protein conformation purified respiratory RNA virus SARS-CoV SARS-CoV, severe acute respiratory syndrome coronavirus sequence similarity staining Support virus [DOI] 10.1016/j.virusres.2014.08.001 PMC 바로가기 [Article Type] Article
Extensive Diversity of Prion Strains Is Defined by Differential Chaperone Interactions and Distinct Amyloidogenic RegionsPrion 균주의 광범위한 다양성은 차등 샤페론 상호 작용 및 고유한 아밀로이드 생성 영역에 의해 정의됩니다Research Article Published on 2014-05-082022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] aggregate aggregates aggregation amyloid Analysis canonical chaperone cofactor cofactors complex conformation conformational conformational change consequence Critical determinant disease disease pathology Disease progression Diseases disorders Diversity domain element examined Extensive Factor help Host Hypothesis induce Interaction involved manifestation modulating molecular neurodegenerative pathology phenotypic phenotypic variation prion progression Propagation Protein Proteins provided PSI Region responsible stability strain Strains structures Support These data Transmission unique Variability variant variants Variation variety widespread yeast [DOI] 10.1371/journal.pgen.1004337 PMC 바로가기 [Article Type] Research Article
Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike ProteinReview Published on 2012-06-202022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] binding cell tropism Characteristics commonality conformational change coronavirus coronavirus S protein Coronaviruses cytoplasm different driven by Entry mechanism feature fusion fusion protein highlight Host host cell host cell membrane Mediated nucleocapsid Pathogenesis pathogenicity primary determinant Proteins proteolytic activation receptor Receptor binding replicate responsible RNA virus spike spike glycoprotein the S protein Transmission Trigger viral entry virus entry [DOI] 10.3390/v4061011 PMC 바로가기 [Article Type] Review
Conformational Reorganization of the SARS Coronavirus Spike Following Receptor Binding: Implications for Membrane FusionResearch Article Published on 2007-10-242022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] ACE2 ACE2 binding Analysis angiotensin-converting enzyme 2 Cell culture change conformational change Cryo-electron microscopy domain fusion in viral initial Isolation membrane fusion molecular morphology occur produced Protein Proteins provide purified receptor Receptor binding reorganization SARS Coronavirus SARS-CoV shown spike subsequent transmembrane domains viral spike virion [DOI] 10.1371/journal.pone.0001082 PMC 바로가기 [Article Type] Research Article