Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA virusesArticle Published on 2005-02-242022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] 229E amplification children detect detected development Efficiency hospitalised human coronavirus human coronavirus OC43 human metapneumovirus immunofluorescence assay included Influenza virus Isolation métapneumovirus multiplex Multiplex assay Multiplex RT-PCR assay nasal parainfluenza virus positive respiratory RNA virus respiratory syncytial virus respiratory viruses rhinovirus RNA RSV RT-PCR RT-PCR assay RT-PCR assays sensitivity TCID50 tested virus [DOI] 10.1016/j.jviromet.2005.01.020 PMC 바로가기 [Article Type] Article
SARS Transmission Pattern in Singapore Reassessed by Viral Sequence Variation AnalysisResearch Article Published on 2005-02-222022-10-29 Journal: PLoS Medicine [Category] Coronavirus, [키워드] accurate acquisition acute respiratory syndrome Analysis approach clinical sample clinical samples Contact demonstrated dependent on detect different Direct effective Epidemiological investigation epidemiological link Epidemiological study finding genetic analysis Genetic variation heterogeneous Host Infectious disease information isolate isolates lung tissues mass molecular nine outbreak overcome pathogen pathogen transmission Patient pattern population level problems provide quasispecy SARS Coronavirus SARS-CoV Sequence analysis sequence variation Singapore Stage thought variety Viral RNA viral sequence virus was determined [DOI] 10.1371/journal.pmed.0020043 PMC 바로가기 [Article Type] Research Article
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infectionsResearch Published on 2005-02-102022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis binding Camelpox cell line cellular compared criteria cytomegalovirus determined experiment expression expression level Gene Human Infected infected cells Infection mRNA expression Protein quantitative real-time PCR ranking Real-time PCR reference reference gene reported SARS Coronavirus tested viral replication virus were infected yellow fever β-actin [DOI] 10.1186/1743-422X-2-7 PMC 바로가기 [Article Type] Research
The Structure of a Rigorously Conserved RNA Element within the SARS Virus GenomeResearch Article Published on 2005-01-282022-10-28 Journal: PLoS Biology [Category] Coronavirus, [키워드] 3′ end absence Anti-viral approach bind Comparisons component conserved coronavirus element explained extreme feature function Genome homology Host host cell host protein Human genome in viral include Interaction interactions interior ions macromolecular mechanism motif mutable occur pathogen pathogenic Protein protein synthesis reactive related required ribosomal RNA RNA RNA genome RNA genomes SARS virus Sequence conservation Structure target therapeutic agents unique Viral RNA virus [DOI] 10.1371/journal.pbio.0030005 PMC 바로가기 [Article Type] Research Article
Molecular mechanisms of severe acute respiratory syndrome (SARS)Review Published on 2005-01-202022-10-28 Journal: Respiratory Research [Category] Coronavirus, [키워드] acute respiratory syndrome approach caused contribute coronavirus Deleterious Effect effort Epidemics Features Genome Health immune immune system indirect effect infections Infectious disease Interaction Laboratory mechanism molecular mechanisms morbidity and mortality mutability Novel coronavirus Occurrence offer Open reading frame outbreak Pathogenesis Prevent pulmonary pathological Region required Research Respiratory disease SARS SARS-CoV severe Severe acute respiratory syndrome target cell the disease therapy vaccination Vaccine viral genome virulence virus widespread [DOI] 10.1186/1465-9921-6-8 PMC 바로가기 [Article Type] Review
Detection of human coronavirus NL63 in young children with bronchiolitisArticle Published on 2005-01-122022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] Acute bronchiolitis acute laryngotracheobronchitis children coronavirus detected detection HCoV‐NL63 Hospitalized immunocompromised adult Inc Infected influenza A less nasopharyngeal swab sample NL63 Pneumonia positive respiratory syncytial virus Respiratory tract infections selected upper respiratory infection virus [DOI] 10.1002/jmv.20289 PMC 바로가기 [Article Type] Article
Monoclonal antibodies versus reverse transcription‐PCR for detection of respiratory viruses in a patient population with respiratory tract infections admitted to hospitalResearch Article Published on 2004-12-152022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] added addition Adults another respiratory virus antibody approach assays association Bronchoalveolar lavage bronchoalveolar lavage sample caused circulating Coinfection Combination coronavirus detected Diagnosis diagnostic examined group H1N1 H3N2 HCoV hospital human coronavirus human metapneumovirus human parainfluenza virus Human respiratory syncytial virus immunological Inc infected children infecting Infection infections Influenza virus investigated less Lower respiratory tract lung transplant majority métapneumovirus molecular Molecular assay monoclonal antibody nasopharyngeal Patient patient population patients peaked pediatric positive proportion recipients respiratory coinfections respiratory infections respiratory syncytial virus respiratory tract infection respiratory viral respiratory virus reverse transcription RT‐PCR RT‐PCR searched Strains tested virus were infected [DOI] 10.1002/jmv.20276 PMC 바로가기 [Article Type] Research Article
SARS Molecular Detection External Quality AssuranceDispatch Published on 2004-12-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] coronavirus detected detection dispatch External NAT network outcome Participants PCR Quality Quality assurance reference RNA copy SARS significantly verification virus were used WHO World Health Organization [DOI] 10.3201/eid1012.040416 PMC 바로가기 [Article Type] Dispatch
Genome structure and transcriptional regulation of human coronavirus NL63Research Published on 2004-11-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] 2003 Analysis Cell culture coding codon usage compared Coronaviral coronavirus detect experimental analysis expression level GC content Genome genome structure HCoV-229E HCoV-NL63 HCoV-OC43 human coronavirus humans increase indicate indicated infect Inspection leader sequence mRNA mRNAs N proteins NL63 Northern blot analysis nucleocapsid nucleotide ORF3 other coronaviruses Pathogenesis pathogenicity performed powerful tool reflected Replication Result RNA genome SARS Coronavirus SARS-CoV sequenced significantly subgenomic mRNA subgenomic mRNAs Transcription transcriptional regulation viral evolution virus [DOI] 10.1186/1743-422X-1-7 PMC 바로가기 [Article Type] Research
Topographic Changes in SARS Coronavirus–infected Cells at Late Stages of InfectionResearch Published on 2004-11-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] Actin active site addition atomic Atomic force microscopy Cell change coronavirus cytoskeletal filament Final infected cell Infection involved maturation Microscopy plasma membrane progeny provided Research SARS coronavirus replication SARS-CoV scanning scanning electron microscopy spike Stage virus virus particle virus particles was used [DOI] 10.3201/eid1011.040195 PMC 바로가기 [Article Type] Research