Culture-free genome-wide locus sequence typing (GLST) provides new perspectives on Trypanosoma cruzi dispersal and infection complexity무배양 게놈 전체 유전자좌 서열 유형 지정(GLST)은 트리파노소마 크루지 분산 및 감염 복잡성에 대한 새로운 관점을 제공합니다.Research Article Published on 2020-12-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] agarose gel alternate allele frequency amplification amplify analyzed approach biases capture capture method Cell Chagas disease Chain Reaction city clone Co-infection collected Complete complex correlation correlations Culture detect dispersal distribution DNA Ecuador epidemiological epidemiological surveillance evaluate Ex vivo flexible frequency distributions generate Genetic Genetic polymorphism Genetic variation Genome genomes genotyping hotspot identify infecting Infection infection source Infectious disease Infectious diseases information intestinal involved library limitation lineages locus marker metagenomic minimum parasite parasites pathogen Pathogenic microorganisms Perspective polymerase chain polymerase chain reaction polymerase chain reactions polymorphism powerful tool primer principle provide purification representing Research Result sequence Sequencing single-nucleotide Single-nucleotide polymorphisms SNP Spectrum Strains subsequent subset Surveillance target target pathogen Trypanosoma Trypanosoma cruzi vector Venezuela [DOI] 10.1371/journal.pgen.1009170 PMC 바로가기 [Article Type] Research Article
Feasibility of continuous fever monitoring using wearable devices웨어러블 기기를 이용한 지속적인 발열 모니터링 가능성Article Published on 2020-12-142022-09-01 Journal: Scientific Reports [Category] MERS, 바이오마커, 진단, [키워드] absence Analysis Biomarker Biotechnology capture collected continuous fever core temperature COVID-19 COVID-19 diagnosis COVID-19 infection COVID-19 infections detect develop Diagnostic markers effort Elevation Fever heterogeneous Hypothesis illness illness onset illnesses MONITOR Novel coronavirus pandemic physiological populations Predictive markers public health reported Research response Scientific data subject Support Symptom temperature These data [DOI] 10.1038/s41598-020-78355-6 PMC 바로가기 [Article Type] Article
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19Multi-Omics는 경증과 중등도의 COVID-19 사이의 급격한 질병 상태 변화를 해결합니다Clinical Trial Published on 2020-12-102022-09-11 Journal: Cell [Category] SARS, 유전자 메커니즘, 임상, [키워드] accompanied amplify Analysis Blood blood clotting blood draw capture changes CITE-seq clinical measurements collected COVID-19 COVID-19 patient Diagnosis disease severity effective elevated feature identify immune Immune cell immune cells immune response Infection inflammatory signaling metabolic processes metabolite metabolites metabolomics Metrics Mild moderate disease multi Multi-omics phenotype plasma proteomics representing SARS-CoV-2 Secretome Sharp shift single-cell single-cell RNA-seq single-cell secretome. single-cell TCR-seq therapeutic intervention [DOI] 10.1016/j.cell.2020.10.037 PMC 바로가기 [Article Type] Clinical Trial
A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2SARS-CoV-2 시퀀싱을 위한 빠르고 비용 효율적인 테일 앰플리콘 분석법Methodology Article Published on 2020-12-042022-09-01 Journal: BMC Genomics [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] amplicon method applied approach approaches balance bypasse capture comparable costly Coverage COVID-19 COVID-19 pandemic diagnostics Genome Genome sequencing genomic isolate isolates labor metagenomic Metrics protocol Result SARS-CoV-2 SARS-CoV-2 sequencing sequence sequenced Sequencing sequencing technology Surveillance time-consuming variant call variant calls Viral Viral surveillance [DOI] 10.1186/s12864-020-07283-6 PMC 바로가기 [Article Type] Methodology Article
Characteristics and short-term obstetric outcomes in a case series of 67 women test-positive for SARS-CoV-2 in Stockholm, Sweden스웨덴 스톡홀름에서 SARS-CoV-2 양성 판정을 받은 여성 67명을 대상으로 한 증례의 특징 및 단기 산과적 결과Article Published on 2020-12-012022-09-11 Journal: Acta obstetricia et gynecologica Scandinavica [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 age antenatal care Asymptomatic Birth calculated capture Care case sery caused Chain Reaction Clinical characteristics clinical presentation Clinical presentations Complication coronavirus coronavirus disease COVID-19 COVID-19 disease COVID-19 illness death deaths delivery outcomes diabete diabetes disease Face mask General population healthy Hospital admission hygiene hypertensive hypertensive disease indicated intensive care majority material maternal deaths medical record medical records mode mother national guideline national guidelines Neonatal neonatal death Neonate Neonates Newborn nulliparous Nursing obese Obstetric occurred outcome outcomes overweight pandemic PCR-positive percentage percentages polymerase chain polymerase chain reaction positive Positive test Pregnancy pregnancy complications pregnancy outcomes Preterm birth preterm births preterm delivery Prevent proportion proportions retrospective SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe coronavirus disease severe COVID-19 illness Stillbirth term tested Viral transmission women [DOI] 10.1111/aogs.14006 PMC 바로가기 [Article Type] Article
Predominance of positive epistasis among drug resistance-associated mutations in HIV-1 proteaseHIV-1 프로테아제의 약물 내성 관련 돌연변이 중 양성 상위성의 우세Research Article Published on 2020-10-212022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] alleviate antiretroviral drug antiretroviral drugs Author capture clinic clinical observation clinical observations Clinical studies clinical study clinically Combination combinations compensatory mutations deep sequencing Deleterious deleterious effect deleterious effects drug drug resistance Effect epistasis Evolution fitness Genetic genetic interactions greatly reduce HIV HIV-1 Impact in vitro inhibitors Interaction interactions involved likelihood long term majority mechanism mutant Mutation mutations observation outcome overcome pandemic patients persistence positive Prophylaxis protease Protease inhibitor protease inhibitors reduce Replication resistance mutations reversal reversion selection experiments sequence Support T cells therapy Viral viral genome viral replication [DOI] 10.1371/journal.pgen.1009009 PMC 바로가기 [Article Type] Research Article
Dynamics of severe acute respiratory syndrome coronavirus 2 genome variants in the feces during convalescence회복기 동안 대변에서 중증 급성 호흡기 증후군 코로나바이러스 2 게놈 변이의 역학Article Published on 2020-10-202022-09-11 Journal: Journal of genetics and genomics = Yi chuan xue ba [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 capture change changes in clinical samples collected convalescent convalescent phase coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated Dynamics Evolution Fecal sample fecal samples feces Frequency gastrointestinal tract genome variant genomic hybrid Hybrid capture individual level individuals Infection Intra-host variant intra-host variants investigated Mutation nine nucleic acid nucleic acids pandemic patients with COVID-19 population level populations random respiratory SARS-CoV-2 SARS-CoV-2 genome severe acute respiratory syndrome Coronavirus Surveillance the SARS-CoV-2 genome Transmission variant Viral viral genome Viral load virus [DOI] 10.1016/j.jgg.2020.10.002 PMC 바로가기 [Article Type] Article
High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing표적 포획 시퀀싱으로 획득한 고범위 SARS-CoV-2 게놈 서열Article Published on 2020-10-012022-09-11 Journal: Journal of medical virology [Category] SARS, 변종, 치료기술, 치료제, [키워드] Biotin can be used capture capture sequencing CDC Cell culture Concentration coronavirus Coronavirus replication cycle threshold demonstrated diagnosed with COVID-19 expression Gene Expression Genetic genetic networks genetic variability genome sequence Health heterozygosity knowledge Map Mutation Nasopharyngeal swab nasopharyngeal swab sample negative controls Patient patients without COVID-19 Phylogenetic relationship positive control probe SARS Coronavirus SARS-CoV-2 SARS-CoV-2 genome sequence SARS-CoV-2 sequence SARS-CoV-2 sequences SARS-CoV-2 viral selected sequence single-stranded DNA subtype swab sample throat swab Viral Viral particles virus detection virus strain [DOI] 10.1002/jmv.26116 PMC 바로가기 [Article Type] Article
High dose dexamethasone treatment for Acute Respiratory Distress Syndrome secondary to COVID-19: a structured summary of a study protocol for a randomised controlled trialCOVID-19에 따른 급성 호흡곤란 증후군에 대한 고용량 덱사메타손 치료: 무작위 대조 시험을 위한 연구 프로토콜의 구조화된 요약Letter Published on 2020-08-262022-08-13 Journal: Trials [Category] MERS, 임상, [키워드] 1:1 active cancer acute respiratory distress acute respiratory distress syndrome Adult patients advance directives Advanced cancer age alpha error anticipated approved ARDS assessment assigned bilateral pulmonary infiltrates Blinding block breast-feeding Buenos Aires capture cardiac disease CEMIC clinical clinical trial code Complete concealed Confirmed COVID-19 COVID-19, Dexamethasone, Acute Respiratory Distress Syndrome, Randomised controlled trial, protocol, Steroids delirium develop Dexamethasone diagnosis of SARS-CoV-2 directive discharge disease dissemination do not resuscitate dose Effectiveness element End-Expiratory Pressure evaluate exclusion criteria Expiratory Pressure explained failure followed by Frequency glucocorticoid Glucocorticoids group hemodynamic High dose HIV infection hypoxemic Hypoxemic respiratory failure ICU Immunosuppression Immunosuppressive agents include inclusion criteria Infection Informed consent intensive care unit International legal guardian Length less Life expectancy local Ethics Committee mechanical ventilation Mortality multicenter muscle weakness Nosocomial infection Nosocomial infections number objective open open label open trial other disease other diseases outcome Palliative care PaO parallel group participant Participation Patient Patient care platform Pneumonia Positive end-expiratory pressure power power of 80% Prednisone pressure Prone position protocol R language random randomised randomised controlled trial randomization Randomized randomized clinical trial receive recruitment Registered renal renal disease respiratory respiratory distress Respiratory distress syndrome Respiratory failure resuscitate order RT-PCR Sample size SARS-COV-2 infection SARS-CoV-2 pneumonia secondary Secondary outcomes secondary to sequence Sequential Organ Failure Assessment severe immunosuppression SOFA Standard deviation status Stratified randomization Study protocol subject Support syndrome table terminal Terminal disease title Treatment treatment groups Trial Trial registration two-tailed alpha error Variation ventilator ventilator-free day Ventilator-free days ventilatory Version viral shedding weakness website women [DOI] 10.1186/s13063-020-04646-y PMC 바로가기 [Article Type] Letter
The effect of prostacyclin (Iloprost) infusion at a dose of 1 ng/kg/min for 72 hours compared to placebo in mechanically ventilated patients with COVID-19: A structured summary of a study protocol for a randomized controlled trial인공호흡기를 사용하는 COVID-19 환자에서 위약과 비교하여 72시간 동안 1ng/kg/min 용량의 프로스타사이클린(일로프로스트) 주입 효과: 무작위 대조 시험을 위한 연구 프로토콜의 구조화된 요약Letter Published on 2020-08-262022-08-13 Journal: Trials [Category] MERS, 임상, [키워드] (Iloprost (normal saline) 1:1 Acute coronary syndrome Adult patient Adult patients age anticipated Biomarker bleeding blinded Blinding block can not Capital Capital Region capture Clinicians comparator concealed conducted Confirmed Confirmed COVID-19 infection Consent coronary syndrome COVID-19 COVID-19 infection COVID-19 patients criteria Day defined Denmark dissemination dose element endothelial endotheliopathy EudraCT exclusion criteria exploratory facilitate FIVE heart failure Hvidovre Hospital hypersensitivity Iloprost intensive care unit intensive care units investigators Local mechanical ventilation mechanically ventilated mechanically ventilated COVID-19 patients mechanically ventilated patient multicenter normal saline number objective outcome parallel group participant Patient patients performed Placebo Pregnancy primary endpoint prostacyclin protocol randomisation randomised Randomized Randomized controlled trial recruitment REDCap Region Sample size sequence statisticians status stratified Study protocol Suspected thrombomodulin total sample size Trial Urine website Withdrawal [DOI] 10.1186/s13063-020-04696-2 PMC 바로가기 [Article Type] Letter