Inefficient Placental Virus Replication and Absence of Neonatal Cell-Specific Immunity Upon Sars-CoV-2 Infection During Pregnancy임신 중 Sars-CoV-2 감염 시 비효율적인 태반 바이러스 복제 및 신생아 세포 특이적 면역 부재Observational Study Published on 2021-06-032022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 변종, 임상, 치료기술, [키워드] acute infection Analysis Asymptomatic Birth Blood children’s health complications consequence convalescent Cord blood Course COVID-19 COVID-19 pandemic described detectable disease during pregnancy Evidence Ex vivo expression higher risk human trial immune immune activation in vitro indicate inefficient infected with SARS-CoV-2 Infection infections inoculation Mild mother Neonatal neonatal infections non-infected observational study Placenta placental Placentas Pregnancy pregnancy complications pregnant pregnant women prenatal infection progression provide rationale recent required risk SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 viral SARS-CoV2 infection severity of COVID-19 T cell T cell response tissues Transmission transmission of SARS-CoV-2 undetectable variants of concern vertical transfer vertical transmission Viral virus virus replication women [DOI] 10.3389/fimmu.2021.698578 PMC 바로가기 [Article Type] Observational Study
Mesenchymal stromal cell delivery via an ex vivo bioreactor preclinical test system attenuates clot formation for intravascular applicationArticle Published on 2021-06-012023-06-19 Journal: Stem cells translational medicine [Category] MERS, SARS, [키워드] bioreactor clot formation time clotting COVID-19 Ex vivo heparin hypercoagulable IBMR instant blood-mediated inflammatory reaction Mesenchymal stromal cells Perfusion [DOI] 10.1002/sctm.20-0454 PMC 바로가기
Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral ParticlesImmunology Published on 2021-05-142022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] 16HBE Act acute respiratory syndrome angiotensin converting enzyme 2 animal models bind binding cells composed coronavirus CRD enzyme epithelial Ex vivo expressing hACE2 HCoV-229E HEK293T cell highlight Human human pulmonary collectins immune in vitro in vivo influenza A inhibited inhibitor innate immune Innate immunity Interaction mucosal surface overexpressing Particle Pathogens previous study protective role pseudotyped lentiviral particle Receptor binding domain reduction in reported respiratory RLU S1 protein SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S1 serum spike spike glycoprotein Spike protein suggested surfactant Surfactant protein D Surveillance the patient therapeutic potential Treatment viral entry viral pathogen [DOI] 10.3389/fimmu.2021.641360 PMC 바로가기 [Article Type] Immunology
The interferon-stimulated exosomal hACE2 potently inhibits SARS-CoV-2 replication through competitively blocking the virus entryArticle Published on 2021-05-122022-10-04 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, SARS, 치료제, [키워드] acute respiratory syndrome Antiviral cell entry cell types coronavirus coronavirus disease COVID-19 Ex vivo exhibiting Exosome expression global pandemic hACE2 human Angiotensin-converting enzyme IFN in vitro indicated inhibit Innate immunity interferon Microbiology outbreak Protein receptor recognize released replication of SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 replication Treatment virus entry [DOI] 10.1038/s41392-021-00604-5 PMC 바로가기 [Article Type] Article
In Vitro Lung Models and Their Application to Study SARS-CoV-2 Pathogenesis and DiseaseReview Published on 2021-04-282022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] air–liquid interface application Cell culture cell culture system Coronaviruses COVID-19 develop disease Efficiency Ex vivo ex vivo lung globe help human airway epithelial cell culture human lung organoids in vitro in vitro lung model lung-on-a-chip mechanisms Model Replication SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 pathogenesis Spread Treatment vaccination [DOI] 10.3390/v13050792 PMC 바로가기 [Article Type] Review
Elderberry for prevention and treatment of viral respiratory illnesses: a systematic review바이러스성 호흡기 질환 예방 및 치료를 위한 엘더베리: 체계적인 검토Research Article Published on 2021-04-072022-08-31 Journal: BMC Complementary Medicine and Therapies [Category] MERS, SARS, 치료제, [키워드] adverse events anti-inflammatory drug benefit Clinical outcome colds common cold Complication conducted COVID-19 COVID-19 pandemic cytokine Cytokine storm Cytokines determine Diclofenac duration of illness effective Elderberry estimate evaluated Evidence Ex vivo FIVE for inclusion GRADE Health illness immune system Impact Inflammation Inflammatory inflammatory markers Influenza information ingestion less lower risk nonsteroidal anti-inflammatory drug not reduce oseltamivir outcomes overproduction Prevent pro-inflammatory cytokines provided randomized trial reduce reduction Research respiratory Respiratory illness respiratory infections respiratory problems Result risk risk of bia Safe Sambucus screened searched severity supplementary material systematic review treat Treatment Viral Viral illness viral respiratory viral respiratory infections [DOI] 10.1186/s12906-021-03283-5 PMC 바로가기 [Article Type] Research Article
T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responsesT 세포 분석은 임상 및 준임상 SARS-CoV-2 감염을 교차 반응성 항바이러스 반응과 구별합니다Article Published on 2021-04-062022-09-10 Journal: Nature Communications [Category] MERS, SARS, 유전자 메커니즘, 임상, 진단, 치료기술, 치료제, [키워드] Antigen antigens antiviral response capture cellular characteristic convalescent COVID-19 immune response cross-reactive cross-reactive immune response Cross-reactive immune responses dependent on ELISPOT Ex vivo healthcare worker ICS identification immune function immune response immune responses Immunity Immunological memory infected with SARS-CoV-2 Infection Lactate Memory responses multiple antigen multiple antigens ORF3 other coronaviruses PCR-confirmed populations pre-existing immunity proliferation Protective Protein response responses S1/S2 SARS-CoV-2 SARS-COV-2 infection seronegative Strong subunit T cell T cell assay T cell assays T cell proliferation T cell response T cell responses understanding uninfected viral infection volunteers [DOI] 10.1038/s41467-021-21856-3 PMC 바로가기 [Article Type] Article
Suppression of SARS-CoV-2 infection in ex-vivo human lung tissues by targeting class III phosphoinositide 3-kinase클래스 III 포스포이노시티드 3-키나아제를 표적으로 하여 생체 외 인간 폐 조직에서 SARS-CoV-2 감염 억제Article Published on 2021-04-012022-09-11 Journal: Journal of medical virology [Category] 신약개발, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus addition Antiviral effect approved ATG14 autophagolysosome autophagy Betacoronavirus canonical caused cellular class III PI3-K clinically complex component coronavirus coronavirus disease COVID-19 COVID-19 treatment Ex vivo ex vivo human lung tissues. examined Factor HCQ Host human lung human lung tissue Immunosuppression include Infection inhibit inhibited inhibiting inhibitor involved kinase Kinase inhibitor lysosomal novel Betacoronavirus pandemic pathway Prevent Previous studies previous study SARS-CoV-2 SARS-COV-2 infection small molecule small molecule inhibitor Small molecule inhibitors Small molecules suggested suppresse suppressed suppression tested therapeutic therapeutic target therapeutic targets tissues Transmissibility Treatment Ulk1 Vps34 [DOI] 10.1002/jmv.26583 PMC 바로가기 [Article Type] Article
Integrin Activation Enables Sensitive Detection of Functional CD4 + and CD8 + T Cells: Application to Characterize SARS-CoV-2 ImmunityImmunology Published on 2021-03-292022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] Activation acute respiratory syndrome Antigen antigen specificity applicability application CD154 CD4 CD4+ T cells CD8 CD8+ T cells cell type clone CMV conformational change coronavirus detection EBV Epstein–Barr virus Ex vivo fluorescent Frequency functional HBV Hepatitis B virus integrin activation intercellular Ligand marker memory MONITOR monoclonal antibody mononuclear cell PBMCs Peripheral blood protocol SARS-CoV-2 sensitive shown T cell T cell responses T cells vaccinated individuals vaccination Whole blood [DOI] 10.3389/fimmu.2021.626308 PMC 바로가기 [Article Type] Immunology
Porcine Hemagglutinating Encephalomyelitis Virus Infection In Vivo and Ex VivoPathogenesis and Immunity Published on 2021-03-242022-10-30 Journal: Journal of Virology [Category] Coronavirus, [키워드] air-liquid interface respiratory epithelial cells Antibody Response Betacoronavirus cause CDCD (cesarean derived change characterized ciliated Clinical sign colostrum deprived) Concentration coronavirus Coronavirus infections Culture cytopathic detection rate did not produce disease Encephalomyelitis epithelia epithelial lining Ex vivo family Coronaviridae ganglia gastritis highest Human IFN-α IL-8 immune response Increased Infection infection process infiltration Inflammatory response inoculated Interferon alpha lesion liver Lungs lymph nodes Macrophage mild respiratory moderate nasal Neonatal neonatal pigs neurological pathogen Pathogenesis plasma platform porcine hemagglutinating encephalomyelitis virus primary site provide provided quantitative PCR respiratory cell respiratory tract RNA robust RT-qPCR secretion T lymphocyte threshold tissue tonsils tracheal tracheobronchial transcriptase upper respiratory tract Viremia virus virus replication vomiting [DOI] 10.1128/JVI.02335-20 PMC 바로가기 [Article Type] Pathogenesis and Immunity